Anesthetic Monitoring: Devices to Use and What the Results Mean

Anesthetic Monitoring: Devices to Use and What the Results Mean
  • 2


Jeff Ko, DVM, MS, Diplomate ACVA, and Rebecca Krimins, DVM

This article is the second in a series discussing the goals of anesthetic monitoring as well as associated procedures and equipment. In the first article, the authors answered questions about anesthetic monitoring, including why it’s performed, information obtained during monitoring, and important components of the anesthetic process.

The main fundamental aspects of anesthetic monitoring are:

  1. Oxygenation (circulatory and respiratory function)
  2. Ventilation (respiratory function)
  3. Circulation (circulatory function with an emphasis on cardiac output).

These three elements work simultaneously in order to maintain adequate tissue and organ perfusion with oxygenated blood.1-3

Oxygenation and ventilation are essential for maintaining a high oxygen level in the blood, while cardiac output plays a pivotal role in maintaining tissue and organ perfusion with highly oxygenated blood. In this way, oxygenation, ventilation, and circulation each play a critical role in providing oxygen to tissues.


Oxygen Delivery

Oxygen delivery is a product of blood oxygenation and cardiac output.1-3

  • Blood oxygenation is represented by blood oxygen content, which is the total amount of oxygen carried by the blood, including oxygen dissolved in plasma and oxygen bound to hemoglobin.1-3
  • Cardiac output is maintained by a complex interaction involving heart rate, stroke volume, peripheral vascular resistance (afterload), blood volume returning to the heart (preload), and blood viscosity.1-3

When heart rate or stroke volume or a combination of both is low, cardiac output is reduced. When blood volume returning to the heart is low due to dehydration or acute blood loss, stroke volume is decreased. If peripheral vascular resistance or blood viscosity (ie, vasoconstriction or polycythemia, respectively) is increased, cardiac output is reduced. In addition, excessively high heart rate (lack of ventricular filling for preload) and profound peripheral vascular dilation (lack of venous return due to peripheral pooling of blood) also reduce cardiac output.

Application to Anesthesia

Acute changes in ventilation or oxygenation can have dramatic effects on oxygen delivery; thus, in addition to monitoring circulatory function (see CIRCULATION, below), it is important to carefully monitor:

  • Saturation level of oxygen in hemoglobin via SaO2 (measured by blood gas analysis) or SpO2 (measured by pulse oximetry)
  • Hematocrit (an important indicator of hemoglobin concentration; hemoglobin concentration equals approximately 1/3 of the hematocrit)
  • Partial pressure of oxygen in arterial blood (PaO2).

When oxygen delivery is inadequate, cells seek alternative ways to supply energy (ATP). The body then converts to anaerobic metabolism, producing lactate as a metabolic by-product and utilizing it as an alternative energy source.4 Measuring a series of blood lactate concentrations provides a trend for assessing tissue perfusion.1-4

Following is a categorization of anesthetic monitoring equipment by physiologic function. See Table 1 and Figure 1 for an outline of anesthetic monitoring equipment and its purposes.




Monitoring circulation during general anesthesia is aided by the use of electrocardiography (ECG) and blood pressure (BP) monitoring as well as patient assessment.


Electrocardiography is easy to perform and should be used continuously during the perioperative period to obtain quick, real-time information about heart rate and rhythm.


The majority of ECGs used in veterinary hospitals have 3 leads and attach to the skin surface.

  • The 3 leads can be placed as either:
    • Limb leads: Left and right forelimb and left hindlimb electrodes attached to the limbs as indicated, with selection of lead II during recording
    • Base-apex leads: Right forelimb and left hindlimb electrodes attached to the right (preferred) or left (alternative) jugular furrow, and left forelimb electrode attached to the opposite side of the thoracic wall caudal to the heart (Figure 2). Select either lead I (negative deflection) or lead III (positive deflection) on the monitor for recording.
  • The advantage of using base-apex lead placement is that it avoids attachment to the hindquarters, minimizing artifact motion during abdominal and hindlimb surgical manipulation.
  • The application of conduction gel and/or medical alcohol on the ECG electrodes minimizes artifact and background electrical noise on the monitor.


ECG indicates electrical activity but does not offer information about the mechanical function of the heart (ie, pumping activity). A form of cardiac arrest called electromechanical dissociation can occur where there is electrical activity of the heart but no pulse present. Therefore, ECG should not be used as a sole monitoring tool for circulatory function perioperatively.

Invasive Blood Pressure Measurement

The gold standard for BP measurement is an arterial catheter connected to a monitor via a pressure transducer (Figure 3). Invasive (direct) BP monitoring provides beat-to-beat information about BP and heart (pulse) rate and rhythm. Arrhythmias can be identified by changes in arterial wave form on the monitor, which may also be accompanied by acute reductions in BP values. Information on arrhythmias diagnosed by ECG, coupled with changes in BP values, allows the anesthetist to determine the severity of the arrhythmias and whether to initiate crucial therapeutic actions.

Arterial catheter placement is usually in the dorsal pedal/metatarsal, femoral, or palmar artery. In dogs with large ears, such as basset hounds, the catheter may be placed in the auricular artery. In cats, arterial catheters are most frequently placed in the dorsal pedal artery.

Advantages and Disadvantages


  • Accurate pressure readings with beat-to-beat information
  • Arterial blood sampling for blood gas analysis, packed cell volume (PCV), glucose, and lactate measurement.


  • Technical skill required to place the catheter
  • Expense of equipment required
  • Frequent maintenance (of pressure transducer, pressure tubing, saline flushing) and calibration
  • Potential hematoma formation and thrombosis (rare)
  • Infection at the catheter insertion site and acute blood loss if an inadvertent disconnection occurs between the catheter and artery (rare).

Noninvasive Blood Pressure Measurement

BP monitoring is more commonly accomplished through noninvasive (indirect) BP monitoring using a Doppler ultrasound or oscillometric method.

Ultrasonic Doppler Method

The ultrasonic Doppler method involves placing a probe on top of an artery with conduction gel applied between the skin and the probe (Figure 4). The probe uses ultrasonic waves to detect pulsatile blood flow or vessel wall motion and converts this to an audible signal.


Any superficial artery can be used (usually limb extremities or ventral tail base) for detection of blood flow. A blood pressure cuff is placed proximal to the ultrasonic probe and is inflated to the point where it exceeds systolic blood pressure, which silences the Doppler signal. The cuff is then gradually deflated until the first noise signal is audible. The blood pressure registered on the sphygmomanometer at this time is the systolic blood pressure.


  • Continuous flow noise is produced.
  • Systolic BP measurement is relatively accurate compared with direct BP monitoring.
  • Equipment cost is low.


  • This method only measures systolic blood pressure.
  • BP has to be measured each time by the anesthetist because the equipment cannot be set for automatic measurements.
  • When vasoconstriction or hypotension occurs, the signal can be relatively weak and difficult to obtain.

Oscillometric Method

Oscillometric BP monitoring (Figure 5) is used to measure systolic, diastolic, and mean arterial BP. Depending on the algorithm used, some devices directly measure systolic and diastolic blood pressure and calculate to yield mean arterial blood pressure, while others measure mean arterial blood pressure directly and then calculate systolic and diastolic blood pressures.


The BP cuff is inexpensive and placed on a limb extremity or the base of the tail and leveled with the heart during measurement. The width of the blood pressure cuff should be 40% of the limb circumference.


  • This method is inexpensive and easy to use.
  • The machine can be set to take automatic measurements at specified time intervals.


  • Technology is motion sensitive and has difficulty reading BP if patient movement, hypotension, bradycardia, or arrhythmias are present.
  • Method is less accurate compared to ultrasonic Doppler and invasive BP monitoring.
  • Variable size and shape of limbs can contribute to inaccuracies, especially in smaller patients (cats and small dogs).

Recent advances in technology are producing improved software and hardware capable of overcoming many of these disadvantages.


Various aspects of respiratory function can be measured using a respirometer, capnometry, and/or blood gas analysis.


Respirometry assesses tidal volume and minute volume in the anesthetized patient. Minute volume (mL/min) is the product of tidal volume (mL) and respiratory rate of the patient:

minute volume = tidal volume × respiratory rate

A respirometer measures expiratory volume and can be placed between the expiratory limb of an anesthetic machine and the anesthetic breathing hose. Alternatively, it can be connected to a tightly-fitting face mask and used to assess ventilation in a nonintubated, sedated patient (Figure 6).


The true usefulness of respirometry is to pinpoint whether respiratory rate and/or tidal volume are inadequate (based on the equation of minute volume) when end-tidal CO2 or PaCO2 is elevated.

Blood Gas Analysis

Arterial blood gas analysis and resultant partial pressure of CO2 in arterial blood (PaCO2) can be used to assess ventilation in the anesthetized patient. The arterial blood sample is collected directly from an artery or through a preplaced arterial catheter (Figure 3).

Although more accurate than a respirometer, PaCO2 measurement requires a blood gas analyzer and arterial blood samples. If arterial blood samples are not available, venous blood samples may be used instead. Results from several human and animal studies show that venous blood sample pH is an acceptable substitute for arterial measurement, and venous CO2 (PvCO2) may be used to detect hypercarbia. However, there may not be sufficient agreement between arterial and venous PCO2 to allow use of venous blood samples for clinical evaluation of ventilatory function.

Handheld blood gas analyzers, such as the iStat system (, VetStat system (, and VitalPath analyzer (, make blood gas analysis more practical and available for veterinary practices.


End-tidal carbon dioxide (ETCO2) monitoring allows exhaled CO2 to be measured noninvasively and reflects PaCO2. Monitoring ETCO2 is useful for:

  • Determining optimal ventilation efficiency
  • Diagnosing respiratory, airway, or device problems, such as apnea, hypoventilation, airway disconnection, airway obstruction, leak in the endotracheal tube cuff, exhaustion of CO2 absorbent, and incompetent one-way valve of anesthetic rebreathing circuit
  • Reflection of adequate cardiac output production (eg, sudden, acute drops in cardiac output are associated with decreases in ETCO2 measurements due to poor pulmonary circulation).

Capnometry is the measurement and numerical display of ETCO2 during the respiratory cycle (inspiration and expiration). A capnometer measures and displays the readings without a graphic presentation; capnography refers to the comprehensive measurement of CO2 using a graphic recorder that displays CO2 concentration in real-time, throughout the respiratory cycle (Figures 7 and 8).


This technique:

  • Is relatively inexpensive.
  • Allows for continuous monitoring of ventilation.
  • Limits the need for invasive procedures, such as arterial blood gas sampling.
  • Provides valuable information on the respiratory status of the patient, including whether the patient has had an inadvertent esophageal intubation.
  • Identifies if the patient is rebreathing carbon dioxide (eg, too much dead space within breathing circuit, inadequate oxygen flow rate used for non-rebreathing circuit, CO2 absorbent exhausted, or a mechanical problem with one-way valve of anesthetic rebreathing circuit).

What the Numbers Mean

ETCO2 measurements usually underestimate actual PaCO2 measurements by 5 to 10 mm Hg. The discrepancy between ETCO2 and PaCO2 measurements is due to alveolar dead space. In the conscious patient, ETCO2 and PaCO2 measurements are very similar. However, the anesthetized patient has increased alveolar dead space, which results in lower ETCO2 measurements.

  • Normal PaCO2 measurements in the anesthetized dog and cat are equal to 35 to 45 mm Hg.
  • ETCO2 (and PaCO2) values higher than 45 mm Hg are consistent with hypoventilation. They alert the anesthetist that the patient may need to breathe more frequently and/or with a larger tidal volume. Hypoventilation is usually associated with anesthetic-induced respiratory depression.
  • ETCO2 (and PaCO2) values lower than 35 mm Hg may be evidence of hyperventilation. Hyperventilation is often associated with pain, light plane of anesthesia, and/or increased body temperature.

For a complete list of normal cardiorespiratory and physiologic parameters in the anesthetized patient, see Table 2.



A patient’s oxygenation status can be measured by blood gas analysis for PaO2 (see Blood Gas Analysis under VENTILATION), hemoximetry, and pulse oximetry. Hemoximetry and pulse oximetry both analyze hemoglobin saturation in oxygen, with hemoximetry providing a more precise measurement. However, it is unusual to use hemoximetry in veterinary practice.

Pulse Oximetry

Pulse oximetry (Figure 9):


  • Calculates the percentage of oxyhemoglobin and reduced hemoglobin present in arterial blood
  • Provides noninvasive, continuous detection of pulsatile arterial blood in the tissue bed (probe is usually attached to the patient’s tongue, lip, ear, or interdigital space)
  • Provides the pulse rate of the patient.

Pulse oximeter function may be affected by many factors, including:

  • Motion artifact (eg, shivering or body movement)
  • Ambient light (eg, fluorescent light affecting proper reading of pulse oximeter)
  • Poor peripheral blood flow due to hypotension or vasoconstriction
  • Electrical noise from surgical instruments (eg, electrocautery)
  • Increased blood carboxyhemoglobin and methemoglobin levels
  • Dark pigmentation of the skin or tongue.

Relationship Between SpO2 and PaO2

  • Normal pulse oximeter readings (SpO2) in anesthetized dogs and cats breathing 100% oxygen should be 98% to 100%.
  • Normal PaO2 in the anesthetized dog and cat breathing 100% oxygen should be greater than 200 mm Hg and can be as high as 650 mm Hg.
  • A SpO2 of 90% corresponds to a PaO2 of 60 mm Hg, which indicates hypoxemia. Hypoxemia is insufficient oxygenation of arterial blood and is considered to be present if:
    • PaO2 is less than or equal to 60 mm Hg
    • SpO2 is less than or equal to 90%.

In the clinical setting, PaO2 can be estimated using pulse oximetry:

PaO2 = SpO2 – 30 (for pulse oximeter readings between 75% and 90%)

This formula only applies to a certain range of pulse oximeter readings because of the linear relationship between the PaO2 and SpO2 values on the mid portion of the hemoglobin disassociation curve. Outside of these values, this rule cannot be applied.


An anesthetic gas analyzer (Figure 8) measures end-tidal inhalant concentration (halothane, isoflurane, sevoflurane, or desflurane). While this value is not a direct representation of the depth of anesthesia, it provides a more precise method for determining the patient’s uptake of anesthetic, in comparison to the vaporizer dial setting.

Minimum alveolar concentration (MAC) is the inhalant anesthetic concentration in the lungs required to prevent purposeful movement in 50% of animals (dogs or cats) in response to surgical (skin incision) stimulus. Understanding MAC in combination with end-tidal anesthetic gas concentration can help the anesthetist avoid too light or deep anesthesia in a patient.

A surgical plane of inhalant anesthesia is usually 1.3 to 1.5 times MAC. Dogs or cats that are maintained on greater than 1.5 to 2 times MAC (one MAC of isoflurane = 1.28% for dogs and 1.63% for cats) of any given inhalant are likely to be in a very deep plane of anesthesia (especially in the premedicated patient). Evaluating MAC can prompt the anesthetist to assess the patient and ensure that the cardiorespiratory functions are appropriate and not severely depressed by the inhalant.


Monitoring body temperature in order to prevent hypothermia and hyperthermia is vital. Body temperature should be monitored perioperatively every 5 to 10 minutes until the patient has recovered to sternal recumbency and body temperature is maintained between 99°F to 102°F.

Hypothermia may result in inability to metabolize anesthetic drugs, prolonged recovery, and impairment of wound healing. Hyperthermia may result in neurologic damage or death if not treated aggressively and in a timely manner; it also results in the patient becoming hypermetabolic, which increases the need for oxygen and glucose.

Body temperature can be monitored continuously with an esophageal temperature probe that measures the temperature of blood flowing through the aorta. Alternatively, a rectal or tympanic temperature can be obtained with a regular thermometer.


Hematocrit & Total Protein

Hematocrit (PCV) not only reflects the blood’s oxygen carrying capacity, in certain surgical cases, monitoring perioperative serial PCVs coupled with serial total protein levels can also aid the anesthetist in proper fluid management, including crystalloid, colloid, and blood transfusion therapy. Anemic patients (perioperative PCV < 20%) have low oxygen content (decreased oxygen carrying capacity due to low hemoglobin concentrations) and, therefore, lower oxygen will be delivered to tissues despite normal cardiac output.

Blood Glucose

Blood glucose monitoring provides vital information about a patient’s glycemic stage. This is particularly important in the pediatric, diabetic, or septic patient. Blood glucose levels that are too high or too low can contribute to prolonged recovery from anesthesia. Monitoring blood glucose levels throughout the perioperative period helps the anesthetist make appropriate decisions regarding dextrose supplementation and/or insulin dosing.

Blood Lactate

Normal lactate concentration is less than 2 mmol/L.4 As previously mentioned, blood lactate concentration will increase if tissue perfusion is inadequate, or if oxygen supply is insufficient to meet the tissue oxygen demand. An elevated lactate level in a patient during the perioperative period requires immediate evaluation of both cardiac output and oxygenation; it also aids the anesthetist in determining appropriate corrective actions.

Lactate is produced when oxygen delivery cannot meet tissue oxygen demands, and the compensatory increase in oxygen extraction is not sufficient to sustain aerobic metabolism. Cells will use anaerobic metabolism as an alternative energy source, producing the by-product lactate.4
Blood lactate concentration can be easily measured with a single drop of blood (Figure 10) using a validated lactate meter for dogs (Lactate Pro hand-held analyser,


Urine Output

Under general anesthesia, normal urine output (1–2 mL/kg/H) represents adequate kidney perfusion and assumes proper perfusion of other organs as well. For patients with renal failure, severe dehydration, or acute hemorrhage, it is important to monitor and check urine output during surgery and recovery. If a urinary catheter is not preplaced, palpation of the animal’s bladder or visual estimation of the volume of urine voided can provide a crude estimate of urine production.

Lack of urine production represents hypoperfusion of the kidneys and other tissues, as well as possible kidney failure. Until adequate urine production is achieved, rehydration and an inotropic agent may be used to treat hypotension or tissue hypoperfusion during anesthesia and the recovery period. Patients that have low urine output with normal tissue perfusion and blood pressure may be in renal failure and might require treatment with diuretics.

The next and final article in this series will discuss corrective actions necessary to address abnormalities detected by anesthetic monitoring equipment in order to achieve the goal of safe anesthesia. 

BP = blood pressure; CO2 = carbon dioxide; ECG = electrocardiography; ETCO2 = end-tidal carbon dioxide; MAC = minimal alveolar concentration; PaCO2 = partial pressure of CO2 in arterial blood; PaO2 = partial pressure of oxygen in arterial blood; PCO2 = partial pressure of CO2; PCV = packed cell volume; PvCO2 = partial pressure of CO2 in venous blood; SaO2 = percentage of available hemoglobin saturated with oxygen; SpO2 = saturation level of oxygen in hemoglobin as measured by pulse oximetry

Read Part 1: Anesthetic Monitoring: Your Questions Answered

Read Part 3: Anesthetic Monitoring: Therapeutic Actions.


  1. Cilley RE, Scharenberg AM, Bongiorno PF, et al. Low oxygen delivery produced by anemia, hypoxia, and low cardiac output. J Surg Res 1991; 51(5):425-433.
  2. Marx G, Reinhart K. Venous oximetry. Curr Opin Crit Care 2006; 12(3):263-268.
  3. Mellema M. Cardiac output, wedge pressure, and oxygen delivery. Vet Clin North Am Small Anim Pract 2001; 31(6):1175-1205.
  4. Allen SE, Holm JL. Lactate: Physiology and clinical utility. J Vet Emerg Crit Care 2008; 18(2):123-132.
  5. Tas O, De Rooster H, Baert E, et al. The accuracy of the Lactate Pro hand-held analyser to determine blood lactate in healthy dogs. J Small Anim Pract 2008; 49(10):504-508.

Jf02_Koeff Ko, DVM, MS, Diplomate ACVA, is a professor in the Department of Veterinary Clinical Sciences at Purdue University College of Veterinary Medicine. He has authored numerous articles and book chapters in the field of anesthesia and pain management. Dr. Ko lectures extensively at regional, national, and international conferences. Dr. Ko can be reached at [email protected]

f02_kriminsRebecca Krimins, DVM, is currently a third-year anesthesia resident in the Department of Veterinary Clinical Sciences at Purdue University College of Veterinary Medicine. Dr. Krimins received her DVM from Ross University School of Veterinary Medicine and her undergraduate degree from University of Chicago. Protection Status
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id='input_6_10_5_label' >ZIP / Postal Code</label> </span><span class='ginput_right address_country' id='input_6_10_6_container' > <select name='input_10.6' id='input_6_10_6' aria-required="true"><option value='' selected='selected'></option><option value='Afghanistan' >Afghanistan</option><option value='Åland Islands' >Åland Islands</option><option value='Albania' >Albania</option><option value='Algeria' >Algeria</option><option value='American Samoa' >American Samoa</option><option value='Andorra' >Andorra</option><option value='Angola' >Angola</option><option value='Anguilla' >Anguilla</option><option value='Antarctica' >Antarctica</option><option value='Antigua and Barbuda' >Antigua and Barbuda</option><option value='Argentina' >Argentina</option><option value='Armenia' >Armenia</option><option value='Aruba' >Aruba</option><option value='Australia' >Australia</option><option value='Austria' >Austria</option><option value='Azerbaijan' >Azerbaijan</option><option value='Bahamas' >Bahamas</option><option value='Bahrain' >Bahrain</option><option value='Bangladesh' >Bangladesh</option><option value='Barbados' >Barbados</option><option value='Belarus' >Belarus</option><option value='Belgium' >Belgium</option><option value='Belize' >Belize</option><option value='Benin' >Benin</option><option value='Bermuda' >Bermuda</option><option value='Bhutan' >Bhutan</option><option value='Bolivia' >Bolivia</option><option value='Bonaire, Sint Eustatius and Saba' >Bonaire, Sint Eustatius and Saba</option><option value='Bosnia and Herzegovina' >Bosnia and Herzegovina</option><option value='Botswana' >Botswana</option><option value='Bouvet Island' >Bouvet Island</option><option value='Brazil' >Brazil</option><option value='British Indian Ocean Territory' >British Indian Ocean Territory</option><option value='Brunei Darussalam' >Brunei Darussalam</option><option value='Bulgaria' >Bulgaria</option><option value='Burkina Faso' >Burkina Faso</option><option value='Burundi' >Burundi</option><option value='Cambodia' >Cambodia</option><option value='Cameroon' >Cameroon</option><option value='Canada' >Canada</option><option value='Cape Verde' >Cape Verde</option><option value='Cayman Islands' >Cayman Islands</option><option value='Central African Republic' >Central African Republic</option><option value='Chad' >Chad</option><option value='Chile' >Chile</option><option value='China' >China</option><option value='Christmas Island' >Christmas Island</option><option value='Cocos Islands' >Cocos Islands</option><option value='Colombia' >Colombia</option><option value='Comoros' >Comoros</option><option value='Congo, Democratic Republic of the' >Congo, Democratic Republic of the</option><option value='Congo, Republic of the' >Congo, Republic of the</option><option value='Cook Islands' >Cook Islands</option><option value='Costa Rica' >Costa Rica</option><option value='Côte d&#039;Ivoire' >Côte d&#039;Ivoire</option><option value='Croatia' >Croatia</option><option value='Cuba' >Cuba</option><option value='Curaçao' >Curaçao</option><option value='Cyprus' >Cyprus</option><option value='Czech Republic' >Czech Republic</option><option value='Denmark' >Denmark</option><option value='Djibouti' >Djibouti</option><option value='Dominica' >Dominica</option><option value='Dominican Republic' >Dominican Republic</option><option value='Ecuador' >Ecuador</option><option value='Egypt' >Egypt</option><option value='El Salvador' >El Salvador</option><option value='Equatorial Guinea' >Equatorial Guinea</option><option value='Eritrea' >Eritrea</option><option value='Estonia' >Estonia</option><option value='Eswatini (Swaziland)' >Eswatini (Swaziland)</option><option value='Ethiopia' >Ethiopia</option><option value='Falkland Islands' >Falkland Islands</option><option value='Faroe Islands' >Faroe Islands</option><option value='Fiji' >Fiji</option><option value='Finland' >Finland</option><option value='France' >France</option><option value='French Guiana' >French Guiana</option><option value='French Polynesia' >French Polynesia</option><option value='French Southern Territories' >French Southern Territories</option><option value='Gabon' >Gabon</option><option value='Gambia' >Gambia</option><option value='Georgia' >Georgia</option><option value='Germany' >Germany</option><option value='Ghana' >Ghana</option><option value='Gibraltar' >Gibraltar</option><option value='Greece' >Greece</option><option value='Greenland' >Greenland</option><option value='Grenada' >Grenada</option><option value='Guadeloupe' >Guadeloupe</option><option value='Guam' >Guam</option><option value='Guatemala' >Guatemala</option><option value='Guernsey' >Guernsey</option><option value='Guinea' >Guinea</option><option value='Guinea-Bissau' >Guinea-Bissau</option><option value='Guyana' >Guyana</option><option value='Haiti' >Haiti</option><option value='Heard and McDonald Islands' >Heard and McDonald Islands</option><option value='Holy See' >Holy See</option><option value='Honduras' >Honduras</option><option value='Hong Kong' >Hong Kong</option><option value='Hungary' >Hungary</option><option value='Iceland' >Iceland</option><option value='India' >India</option><option value='Indonesia' >Indonesia</option><option value='Iran' >Iran</option><option value='Iraq' >Iraq</option><option value='Ireland' >Ireland</option><option value='Isle of Man' >Isle of Man</option><option value='Israel' >Israel</option><option value='Italy' >Italy</option><option value='Jamaica' >Jamaica</option><option value='Japan' >Japan</option><option value='Jersey' >Jersey</option><option value='Jordan' >Jordan</option><option value='Kazakhstan' >Kazakhstan</option><option value='Kenya' >Kenya</option><option value='Kiribati' >Kiribati</option><option value='Kuwait' >Kuwait</option><option value='Kyrgyzstan' >Kyrgyzstan</option><option value='Lao People&#039;s Democratic Republic' >Lao People&#039;s Democratic Republic</option><option value='Latvia' >Latvia</option><option value='Lebanon' >Lebanon</option><option value='Lesotho' >Lesotho</option><option value='Liberia' >Liberia</option><option value='Libya' >Libya</option><option value='Liechtenstein' >Liechtenstein</option><option value='Lithuania' >Lithuania</option><option value='Luxembourg' >Luxembourg</option><option value='Macau' >Macau</option><option value='Macedonia' >Macedonia</option><option value='Madagascar' >Madagascar</option><option value='Malawi' >Malawi</option><option value='Malaysia' >Malaysia</option><option value='Maldives' >Maldives</option><option value='Mali' >Mali</option><option value='Malta' >Malta</option><option value='Marshall Islands' >Marshall Islands</option><option value='Martinique' >Martinique</option><option value='Mauritania' >Mauritania</option><option value='Mauritius' >Mauritius</option><option value='Mayotte' >Mayotte</option><option value='Mexico' >Mexico</option><option value='Micronesia' >Micronesia</option><option value='Moldova' >Moldova</option><option value='Monaco' >Monaco</option><option value='Mongolia' >Mongolia</option><option value='Montenegro' >Montenegro</option><option value='Montserrat' >Montserrat</option><option value='Morocco' >Morocco</option><option value='Mozambique' >Mozambique</option><option value='Myanmar' >Myanmar</option><option value='Namibia' >Namibia</option><option value='Nauru' >Nauru</option><option value='Nepal' >Nepal</option><option value='Netherlands' >Netherlands</option><option value='New Caledonia' >New Caledonia</option><option value='New Zealand' >New Zealand</option><option value='Nicaragua' >Nicaragua</option><option value='Niger' >Niger</option><option value='Nigeria' >Nigeria</option><option value='Niue' >Niue</option><option value='Norfolk Island' >Norfolk Island</option><option value='North Korea' >North Korea</option><option value='Northern Mariana Islands' >Northern Mariana Islands</option><option value='Norway' >Norway</option><option value='Oman' >Oman</option><option value='Pakistan' >Pakistan</option><option value='Palau' >Palau</option><option value='Palestine, State of' >Palestine, State of</option><option value='Panama' >Panama</option><option value='Papua New Guinea' >Papua New Guinea</option><option value='Paraguay' >Paraguay</option><option value='Peru' >Peru</option><option value='Philippines' >Philippines</option><option value='Pitcairn' >Pitcairn</option><option value='Poland' >Poland</option><option value='Portugal' >Portugal</option><option value='Puerto Rico' >Puerto Rico</option><option value='Qatar' >Qatar</option><option value='Réunion' >Réunion</option><option value='Romania' >Romania</option><option value='Russia' >Russia</option><option value='Rwanda' >Rwanda</option><option value='Saint Barthélemy' >Saint Barthélemy</option><option value='Saint Helena' >Saint Helena</option><option value='Saint Kitts and Nevis' >Saint Kitts and Nevis</option><option value='Saint Lucia' >Saint Lucia</option><option value='Saint Martin' >Saint Martin</option><option value='Saint Pierre and Miquelon' >Saint Pierre and Miquelon</option><option value='Saint Vincent and the Grenadines' >Saint Vincent and the Grenadines</option><option value='Samoa' >Samoa</option><option value='San Marino' >San Marino</option><option value='Sao Tome and Principe' >Sao Tome and Principe</option><option value='Saudi Arabia' >Saudi Arabia</option><option value='Senegal' >Senegal</option><option value='Serbia' >Serbia</option><option value='Seychelles' >Seychelles</option><option value='Sierra Leone' >Sierra Leone</option><option value='Singapore' >Singapore</option><option value='Sint Maarten' >Sint Maarten</option><option value='Slovakia' >Slovakia</option><option value='Slovenia' >Slovenia</option><option value='Solomon Islands' >Solomon Islands</option><option value='Somalia' >Somalia</option><option value='South Africa' >South Africa</option><option value='South Georgia' >South Georgia</option><option value='South Korea' >South Korea</option><option value='South Sudan' >South Sudan</option><option value='Spain' >Spain</option><option value='Sri Lanka' >Sri Lanka</option><option value='Sudan' >Sudan</option><option value='Suriname' >Suriname</option><option value='Svalbard and Jan Mayen Islands' >Svalbard and Jan Mayen Islands</option><option value='Sweden' >Sweden</option><option value='Switzerland' >Switzerland</option><option value='Syria' >Syria</option><option value='Taiwan' >Taiwan</option><option value='Tajikistan' >Tajikistan</option><option value='Tanzania' >Tanzania</option><option value='Thailand' >Thailand</option><option value='Timor-Leste' >Timor-Leste</option><option value='Togo' >Togo</option><option value='Tokelau' >Tokelau</option><option value='Tonga' >Tonga</option><option value='Trinidad and Tobago' >Trinidad and Tobago</option><option value='Tunisia' >Tunisia</option><option value='Turkey' >Turkey</option><option value='Turkmenistan' >Turkmenistan</option><option value='Turks and Caicos Islands' >Turks and Caicos Islands</option><option value='Tuvalu' >Tuvalu</option><option value='Uganda' >Uganda</option><option value='Ukraine' >Ukraine</option><option value='United Arab Emirates' >United Arab Emirates</option><option value='United Kingdom' >United Kingdom</option><option value='United States' >United States</option><option value='Uruguay' >Uruguay</option><option value='US Minor Outlying Islands' >US Minor Outlying Islands</option><option value='Uzbekistan' >Uzbekistan</option><option value='Vanuatu' >Vanuatu</option><option value='Venezuela' >Venezuela</option><option value='Vietnam' >Vietnam</option><option value='Virgin Islands, British' >Virgin Islands, British</option><option value='Virgin Islands, U.S.' >Virgin Islands, U.S.</option><option value='Wallis and Futuna' >Wallis and Futuna</option><option value='Western Sahara' >Western Sahara</option><option value='Yemen' >Yemen</option><option value='Zambia' >Zambia</option><option value='Zimbabwe' 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