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Parasitology

Chagas Disease in Dogs: Transmission, Diagnosis, Treatment, and Prevention

Triatomine insect vectors, which spread Chagas disease to dogs and humans alike, are prevalent in the southern U.S. and Latin America.

Ashley B. Saunders DVM, DACVIM (Cardiology)

Ashley B. Saunders, DACVIM (Cardiology), is a professor of cardiology in the Department of Small Animal Clinical Sciences at Texas A&M University College of Veterinary Medicine and Biomedical Sciences. Her interests include interventional cardiology and advanced imaging, heart failure management, and educational technology. She is the recipient of multiple teaching awards and is a Montague Center for Teaching Excellence Scholar.

Sarah A. HamerMS, PhD, DVM, DACVPM (Epidemiology)

Dr. Hamer is a veterinary ecologist and epidemiologist at the Texas A&M University College of Veterinary Medicine and Biomedical Sciences. She runs a research laboratory focused on ecology of emerging zoonotic and vector-borne diseases at the human-wildlife-domestic animal interface.

Chagas Disease in Dogs: Transmission, Diagnosis, Treatment, and Prevention
Courtesy Gabriel Hamer/TAMU Entomology
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Chagas disease is caused by Trypanosoma cruzi, a protozoal organism primarily transmitted by triatomine insect vectors, also known as “kissing bugs.” It is a zoonotic disease originally described by Brazilian physician Dr. Carlos Chagas in 1909 and is widespread in Latin America. Although triatomines and T. cruzi have long been endemic to the southern United States, awareness and identification of infected vectors and animals have recently increased throughout the United States. Canine Chagas disease can be acute or chronic and is predominantly characterized by inflammation and fibrosis of the heart, resulting in arrhythmias, myocardial dysfunction, heart failure, and sudden death, although many infected dogs are asymptomatic.

TRIATOMINE VECTORS

Triatomine insect vectors belong to the family Reduviidae, subfamily Triatominae (Figure 1). In the southern United States, 11 triatomine species are established; species regularly encountered by people and dogs include Triatoma gerstaeckeri, Triatoma sanguisuga, Triatoma rubida, and Triatoma recurva (Figure 2).1 In contrast to the domesticated species found in Central and South America, which live in human dwellings, many triatomines of the southern United States are considered to be sylvatic (associated with wildlife and natural habitats) and only occasionally drawn into the domestic and peridomestic environments. Colonization of homes by triatomines in the United States is often associated with rustic or dilapidated housing, or with the T. recurva species in Arizona.2

Triatomine insects feed primarily on blood. They are generalist feeders and obtain blood meals from many hosts, predominantly vertebrates (e.g., wild/domestic mammals, birds, amphibians, reptiles, humans); they have also been shown to feed on invertebrates
(e.g., crickets).

Infection prevalence of T. cruzi in triatomines ranges from approximately 20% to 70% depending on species, with an overall 54% infection prevalence reported across specimens from 17 states (predominantly Texas) submitted to a Kissing Bug Citizen Science program.3

Risk factors for T. cruzi infection include living in or traveling to an area with infected insect vectors.4 All dogs are at risk for infection; however, dogs with lifestyles that include outdoor work or housing can have increased risk of exposure.4-6 Dog kennels may be particularly suitable environments for the establishment of T. cruzi transmission cycles. High densities of dogs in confined areas are associated with heat and carbon dioxide, which attract kissing bugs. For example, nearly 40% of triatomines collected by members of the public and submitted to a Kissing Bug Citizen Science program were collected from kennel environments.3

Several insects appear similar to the kissing bug and cause confusion among the public and veterinary community (Box 1). These “look-alike” insects do not feed on blood from humans or animals (although some are associated with painful bites) and pose no risk for the transmission of T. cruzi.

BOX 1 Look-Alike Species Commonly Mistaken For Kissing Bugs
Some of the most common insects that look similar to kissing bugs, yet pose no risk of T. cruzi transmission, include (A) the wheel bug, (B) Zelus longipes, and (C) Leptoglossus brevirostris. For help identifying kissing bugs, or to submit a triatomine for free Trypanosoma cruzi testing, please visit the Texas A&M University Kissing Bug Citizen Science Program website (kissingbug.tamu.edu). Photo courtesy Mike Merchant/TAMU Entomology (3).

TRYPANOSOMA CRUZI

T. cruzi Life Cycle

The morphologic forms of the T. cruzi parasite include amastigotes, epimastigotes, and trypomastigotes. Amastigotes are found in mammalian host cells, and epimastigotes live in the hindgut of the triatomine insect. Both replicate by binary fission. Trypomastigotes are present in the bloodstream of mammalian hosts during acute infection and can be seen in blood smears (Figure 3). Infective trypomastigotes are also present in the hindgut and feces of the triatomine insect vector.

T. cruzi Transmission

The T. cruzi parasite is transmitted through the stercorarian (vector-fecal) route when a kissing bug excretes the parasite in its fecal material onto the host and the parasite enters through the bite wound, a break in the skin, or a mucous membrane. Although this method of transmission is inefficient,7 an epidemiologic setting with insects that colonize the home and feed night after night is a high-risk scenario for vector-fecal transmission to humans. Oral transmission can occur after consumption of fruit or juices contaminated with triatomine feces or through consumption of infected insects, as has been shown in experimental lab studies with raccoons.8 The relative importance of stercorarian versus oral transmission in dogs is unknown.

Nonvector routes of parasite transmission include transplacental and transmammary from infected mother to offspring (human, animal), blood transfusion (human), ingestion of an infected animal (human, animal),9 and rare laboratory accident (human). In pregnant women with antibodies to T. cruzi, the global rate of transmission is estimated to be 4.7%,10 while a corresponding estimate for veterinary congenital transmission is unknown.

In nature, many wild and domestic animals are involved in maintaining the T. cruzi transmission cycle. Hundreds of mammalian species, including dogs, can become infected with T. cruzi. Birds, reptiles, and amphibians, however, are incompetent hosts. The most commonly studied T. cruzi hosts in the southern United States include raccoons, woodrats, opossums, and dogs.11 Domestic cats are increasingly recognized as infected and potentially clinically affected hosts.12 The relative importance of different reservoir hosts—that is, species that become infected and serve as a source of infection to feeding triatomines—likely varies geographically. In South America, dogs and cats are recognized as parasite reservoirs,13 but their importance as reservoirs in the United States is unknown.

T. cruzi Prevalence

Prevalence of T. cruzi infection among dogs ranges depending on canine population and type of serologic test used. For example, antibodies against T. cruzi were detected in 18% and 7% of shelter dogs in Texas and Louisiana, respectively.14,15 In a study of dogs housed in kennels in central Texas where triatomines were abundant, seroprevalence exceeded 50%.16

CHAGAS DISEASE IN DOGS

Chagas Disease Distribution

Chagas disease is prevalent in Latin America and is increasingly recognized in the United States. In areas where triatomines are established, infections may be acquired from local vectors.17 However, Chagas disease is not geographically confined to regions where triatomines live, so human medical and veterinary providers may encounter patients with Chagas disease outside traditionally endemic environments.18 Travel and relocation of animals have the potential to redistribute infected dogs; for example, in one study, government working dogs that provide security and border protection in northern states tested positive for T. cruzi, likely reflecting infections acquired when the dogs trained in southern states.19 Consequently, Chagas disease should be considered in the differential diagnosis for unexplained arrhythmias and myocardial dysfunction in dogs with unknown origin or travel history or known travel to endemic regions.

Clinical Presentation of Chagas Disease in Dogs

The progression of Chagas disease in dogs is similar to that in humans. Inflammation, fibrosis, and cellular damage are caused by trypomastigotes invading host cells to become pseudocysts of intracellular amastigotes. Inflammation is more pronounced in the acute stage, developing into fibrosis in the chronic stage, at which point amastigotes are much less likely to be identified. While the heart is the organ primarily affected in dogs, parasites can be found in many other tissues (e.g., liver, kidney, spleen, brain, skeletal muscle, lymph node).20,21 Information about progression of disease in naturally infected dogs is expanding with continued study.22

Acute disease develops within approximately 21 days of infection, followed by an indeterminate, asymptomatic phase that can progress into chronic disease. In experimental T. cruzi infections, dogs developed chronic disease 8 to 36 months after inoculation.23 Dogs with acute and chronic disease often remain undiagnosed and are asymptomatic or have only mild clinical signs such as fever, lethargy, lymph node enlargement, and organomegaly (spleen, liver).21,23

Cardiac abnormalities develop in both acute and chronic disease, with damage resulting in arrhythmias, conduction abnormalities, heart enlargement, myocardial dysfunction, heart failure, and sudden death.4,20,22-24 Clinical signs include weakness, lethargy, collapse, and signs of congestive heart failure (e.g., abdominal distention, respiratory difficulty).4,21,23,24 Physical examination findings support the presence of heart disease, characterized most often as tachycardia/bradycardia, weak or irregular pulses, murmur, dyspnea, and ascites.

Diagnosis of Chagas Disease in Dogs

Chagas disease is a diagnostic differential for dogs with arrhythmias, myocardial dysfunction, and congestive heart failure. It can present with findings similar to those of dilated cardiomyopathy (idiopathic, nutritional deficiency), arrhythmogenic right ventricular cardiomyopathy, other forms of infectious myocarditis, and congenital tricuspid valve dysplasia.20 The index of suspicion for Chagas disease increases in dogs from an endemic location or that have originated from, traveled to, or are currently living in an area where Chagas disease has been reported.4 Testing should be considered for dogs with a mother, littermate, or housemate (dog or cat) that has tested positive for T. cruzi infection. As there is no gold standard test, the sensitivity and specificity of the available tests are unknown.

Microscopy

Identification of trypomastigotes on a buffy coat or whole blood smear (Figure 3) can enable a positive diagnosis of an acute infection when serologic test results are negative early in the disease process. Trypomastigotes and amastigotes can be identified during thorough microscopic examination of needle aspirates of enlarged lymph nodes or, more often, on histopathologic sections of the heart (Figure 4) or other organs (e.g., spleen, liver, kidney, lymph node, diaphragm).20,21

Serology

Indirect fluorescent antibody testing, currently available at the Texas A&M Veterinary Medical Diagnostic Laboratory, is the most accessible test for T. cruzi infection in dogs. It confirms exposure to the parasite, often indicating current infection because infections are thought to be lifelong.23 A correlation between titer result and clinical disease has not been established. False negatives are possible during an acute infection within the first month of exposure, and cross-reactivity with Leishmania is possible. In human medicine, diagnosis of Chagas disease is a challenge, and multiple independent testing platforms are commonly used to look for consensus in results.17 Rapid tests that detect antibodies to T. cruzi for the diagnosis of Chagas disease in humans are not currently approved for clinical use in animals.

Polymerase Chain Reaction Testing

Polymerase chain reaction (PCR) testing is used to detect parasite DNA in blood and tissue.25 Antemortem testing of blood samples can detect circulating trypomastigotes more likely to be present in acute infections, but test results are limited by the amount of circulating organism present. In chronic disease, PCR results are limited by the low probability that a dog has circulating trypomastigotes. For these reasons, a negative PCR result should not be interpreted to indicate that the host is uninfected and alone is of limited utility as a test of cure. Postmortem PCR testing can be useful to confirm the presence of the organism in tissue samples (e.g., cardiac tissue).

Medical Tests for Heart Disease

Diagnostic tests for heart disease include electrocardiography (ECG), echocardiography, and cardiac troponin I. Screening for heart disease in asymptomatic dogs provides information about heart function and arrhythmias that may be clinically silent but increase the risk of clinical signs and sudden death. In dogs with T. cruzi infection, ECG abnormalities vary widely based on the location and extent of myocardial damage and resultant inflammation and fibrosis but include changes to the ECG complex, conduction disturbances (atrioventricular block, bundle branch block), and both brady- and tachyarrhythmias.4,20,21,24 Infected dogs are more likely to have ventricular arrhythmias and combinations of ECG abnormalities.4 An ambulatory ECG (Holter monitor) recording can detect arrhythmias not present during physical examination.4

A recent study using Holter monitoring of serologically positive and negative working dogs along the Texas-Mexico border showed that 78% of positive dogs and 11% of negative dogs had ECG abnormalities, most commonly supraventricular and ventricular arrhythmias and atrioventricular block.22

Echocardiographic abnormalities include enlargement of all 4 heart chambers and ventricular systolic dysfunction. Elevations in cardiac troponin I, a nonspecific indicator of myocardial damage, are reported in dogs infected with T. cruzi.4,22

Treatment of Chagas Disease in Dogs

Antiprotozoal treatment protocols are not well established in dogs. Prospective studies that include risk-benefit assessment and further investigation into optimal timing and dose of medications are needed to help improve the long-term clinical course of Chagas disease. Anti-trypanosomal medications (e.g., benznidazole, nifurtimox, itraconazole) used specifically to treat T. cruzi infection may show promise but do not currently have consistently proven efficacy for curing Chagas disease in dogs or preventing the chronic fibrosis and progressive myocardial dysfunction that develops in dogs or humans.17,26 These medications have been associated with temporary suppression of parasitemia and have a range of side effects.17 Additionally, treatment response in dogs may be T. cruzi strain dependent.26

Benznidazole treatment in humans and dogs has shown variable clinical benefit and a high possibility of adverse effects, and the medication is not readily available for dogs in the United States.17,26 The combined use of itraconazole and the antiarrhythmic medication amiodarone has been described in the treatment of serologically positive dogs.27

Although complex, additional prospective investigation to determine therapeutic protocols, evaluate adverse effects, and establish diagnostic test strategies to assess potential cure and clinical response in symptomatic and asymptomatic naturally infected dogs would be useful.

Medical therapy is tailored to the clinical abnormalities identified in an individual dog, which are most often related to the heart. These include antiarrhythmics for tachyarrhythmias, pacemaker implantation for bradyarrhythmias, positive inotropic therapy with pimobendan for myocardial dysfunction, and diuretic therapy for heart failure.20 Symptomatic chronic disease requires long-term management.

Chagas Disease Prevention for Dogs

Vector control is an important part of managing disease transmission. Kissing bug habitats include wooded areas, brush piles, nests, and porches.1 The insects are most active at night, are attracted to lights, and can invade homes and kennels. Vector control can include turning off outdoor lights, cleaning up brush and woodpiles that serve as breeding areas for insects, housing animals inside, and using insecticides. Kissing bug control can be difficult in kennels, particularly in recently developed areas where kennels are surrounded by natural habitats with associated wildlife.

Improving public awareness for dog owners and medical professionals is an important step in infection prevention, vector control, and identification of infected animals. Because of its zoonotic potential, it is important for personnel handling blood and tissue of infected animals to take appropriate safety precautions.

CONCLUSION

Although the insects that transmit T. cruzi are primarily reported in the southern half of the United States and across Latin America, any dog with vector contact can be infected, including dogs that live in northern states and have a travel history. Infected animals can be asymptomatic or develop a range of cardiac complications, including sudden death or chronic disease. Because of the challenges in diagnosis and lack of well-established antiparasitic treatments, Chagas disease is an active area of scientific study.

References

1. Curtis-Robles R, Hamer S, Lane S, et al. Bionomics and spatial distribution of Triatomine vectors of Trypanosoma cruzi in Texas and other southern states, USA. Am J Trop Med Hyg 2018;98(1):113-121.

2. Behrens-Bradley N, Smith S, Beatty NL, et al. Kissing bugs harboring Trypanosoma cruzi frequently bite residents of the US Southwest but do not cause Chagas disease. Am J Med 2020;133(1):108-114.e13.

3. Curtis-Robles R, Auckland LD, Snowden KF, et al. Analysis of over 1500 triatomine vectors from across the US, predominantly Texas, for Trypanosoma cruzi infection and discrete typing units. Infec Genet Evol 2018;58:171-180.

4. Meyers AC, Hamer SA, Matthews DM, et al. Risk factors and select cardiac characteristics associated with Trypanosoma cruzi infection in naturally infected dogs presenting to a teaching hospital in Texas.
J Vet Intern Med 2019;33(4):1695-1706.

5. Kjos SA, Snowden KF, Craig TM, et al. Distribution and characterization of canine Chagas disease in Texas. Vet Parasitol 2008;152:249-256.

6. Meyers AC, Meinders M, Hamer SA. Widespread Trypanosoma cruzi infection in government working dogs along the Texas-Mexico border: discordant serology, parasite genotyping and associated vectors.
PLoS Negl Trop Dis 2017;11(8):e1-19.

7. Nouvellet P, Dumonteil E, Gourbière S. The improbable transmission of Trypanosoma cruzi to human: the missing link in the dynamics and control of Chagas disease. PLoS Negl Trop Dis 2013;7(11):e2505.

8. Roellig DM, Ellis AE, Yabsley MJ. Oral transmission of Trypanosoma cruzi with opposing evidence for the theory of carnivory. J Parasitol 2009;95(2):360-364.

9. Pereira KS, Schmidt FL, Guaraldo AMA, et al. Chagas’ disease as a foodborne illness. J Food Prot 2009;72(2):441–446.

10. Howard E, Xiong X, Carlier Y, et al. Frequency of the congenital transmission of Trypanosoma cruzi: a systematic review and meta-analysis. BJOG 2014;121(1):22–33.

11. Hodo CL, Hamer SA. Toward an ecological framework for assessing reservoirs of vector-borne pathogens: wildlife reservoirs of Trypanosoma cruzi across the southern United States. ILAR J 2017;58(3):379-392.

12. Zecca IB, Hodo CL, Slack S, et al. Prevalence of Trypanosoma cruzi infection and associated histologic findings in domestic cats (Felis catus). Vet Parasitol 2019;278:109014.

13. Gürtler RE, Cecere MC, Lauricella MA,
et al. Domestic dogs and cats as sources of Trypanosoma cruzi infection in rural northwestern Argentina. Parasitology 2007;134(Pt 1):69-82.

14. Hodo CL, Rodriguez JY, Cutis-Robles R, et al. Repeated cross-sectional study of Trypanosoma cruzi in shelter dogs in Texas, in the context of Dirofilaria immitis and tick-borne pathogen prevalence. J Vet Intern Med 2019;33(1):158-166.

15. Elmayan A, Tu W, Duhon B, et al. High prevalence of Trypanosoma cruzi infection in shelter dogs from southern Louisiana, USA. Parasit Vectors 2019;12(1):322.

16. Curtis-Robles R, Snowden KF, Dominguez B, et al. Epidemiology and molecular typing of Trypanosoma cruzi in naturally-infected hound dogs and associated triatomine vectors in Texas, USA. PLoS Neg Trop Dis 2017;11(1):e0005298.

17. Bern C, Messenger LA, Whitman JD, Maguire JH. Chagas disease in the United States: a public health approach. Clin Microbiol Rev 2019;33(1):456-442.

18. Nunes MCP, Beaton A, Acquatella H, et al. Chagas cardiomyopathy: an update of current clinical knowledge and management: A scientific statement from the American Heart Association. Circulation 2018;138(12):e169-209.

19. Meyers AC, Purnell JC, Ellis MM, et al. Nationwide exposure of U.S. working dogs to the Chagas disease parasite, Trypanosoma cruzi. Am J Trop Med Hyg
2020;102(5):1078-1085.

20. Vitt JP, Saunders AB, O’Brien MT, et al. Diagnostic features of acute Chagas myocarditis with sudden death in a family of boxer dogs. J Vet Intern Med
2016;30(4):1210-1215.

21. Nabity M, Barnhart K, Logan KS, et al. An atypical case of Trypanosoma cruzi infection in a young English mastiff. Vet Parasitol 2006;140(3-4):356-361.

22. Meyers AC, Ellis MM, Purnell JC, et al. Selected cardiac abnormalities in Trypanosoma cruzi serologically positive, discordant, and negative working dogs along the Texas-Mexico border. BMC Vet Res 2020;16(1):101.

23. Barr SC. Canine Chagas’ disease (American Trypanosomiasis) in North America. Vet Clin North Am Small Anim Pract
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24. Saunders AB, Gordon SG, Rector MH, et al. Bradyarrhythmias and pacemaker therapy in Chagas positive dogs. J Vet Intern Med 2013;27(4):890-894.

25. Araujo FM, Bahia MT, Magalhaes NM, et al. Follow-up of experimental chronic Chagas’ disease in dogs: Use of polymerase chain reaction (PCR) compared with parasitological and serological methods. Acta Trop 2002;81(1):21-31.

26. Cunha ELA, Torchelsen FKVDS, Cunha LM, et al. Benznidazole, itraconazole and their combination in the treatment of acute experimental Chagas disease in dogs. Exp Parasitol 2019;204:107711.

27. Madigan R, Majoy S, Ritter K, et al. Investigation of a combination of amiodarone and itraconazole for treatment of American trypanosomiasis (Chagas disease) in dogs. JAVMA 2019;255(3):317-329.

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