Challenges & New Developments in Canine Pyoderma: Disease Overview & Diagnosis




Kimberly S. Coyner, DVM, Diplomate ACVD

This overview of canine pyoderma discusses disease transmission, underlying conditions, clinical signs, differentials, and diagnostics. Future articles in this series will address treatment and methicillin resistance.

This is the first article in a 3-part series discussing the latest information available regarding canine pyoderma. Future articles will discuss treatment as well as methicillin-resistant infection.

  • Canine pyoderma is a bacterial skin disease usually caused by Staphylococcus pseudintermedius (previously known as Staphylococcus intermedius).1
  • S pseudintermedius is a gram-positive, coagulase-positive cocci bacteria, which is considered part of the normal canine mucosal flora and cultured from 37% to 41% of normal dogs.2,3
  • Less common bacterial species found in canine pyodermas include S aureus (4.7%–8.3% of cases, usually human origin) and S schleiferi (19%–28% of cases).4-6

The Three Ms: MIC, MPC, & MSW

Mean inhibitory concentration (MIC) is usually based on blood levels of an antibiotic, and is the minimal antibiotic concentration needed to inhibit bacterial growth. When the MIC exceeds the concentration of drug that can be safely achieved in the bloodstream, then the organism is deemed resistant.

Bacterial Mutation & Resistance

MIC is based on standardized bacterial inoculums (105 CFU/mL) exposed to varying drug concentrations in a test tube, and it varies with both the drug and the bacterial species targeted.

  • Since MIC does not equal complete killing of all bacteria, bacterial mutation and resistance is a concern.
  • Patients with normal intact immune systems: Inhibition of the susceptible bacterial population allows immune clearance of infection, including resistant mutants.
  • Immunocompromised patients, those with prior infection or previous exposure to antibiotics, or those in which therapy for acute infection fails: Continued proliferation of resistant mutants may occur.
  • When a high-density bacterial population is exposed to an antimicrobial agent, it only requires one spontaneous mutation to the exposed agent for the culture to become a > 1012 population of resistant bacteria following overnight incubation.1

Measuring Mutant Prevention

Recently, the mutant prevention concentration (MPC) has been described as a novel measurement of in vitro bacterial susceptibility or resistance. MPC defines the lowest drug concentration required to block the growth of the least susceptible bacterium present in the tested population.

MPC is based on the testing of a larger bacterial inoculum (>= 109 CFU/mL), which:

  • More closely approximates bacterial load in actual infections
  • Takes into account the probability of mutant subpopulations being present in high-density bacterial populations.1,2

Dosing based on MPC drug concentration may reduce overall bacterial numbers as well as prevent the selective amplification of the resistant subpopulation. As with MIC, MPC varies with both the antimicrobial and bacterial species targeted.

Minimizing Mutation Development

The mutant selection window (MSW) defines the danger zone for therapeutic drug concentrations, and is bordered by the MIC and the MPC values. Minimizing the length of time that an antibiotic concentration remains in the MSW may reduce the likelihood for development of resistance during therapy.

Achieving Optimal Antibiotic Dosing

MPC values, when considered with antimicrobial pharmacology, may allow more accurate prediction of probability of resistance when bacteria are exposed to antibiotics during therapy for infectious diseases. With the increasing emergence of resistant infections, resistance prevention should be an important goal of antimicrobial therapy.1-3
Hopefully, MPC and MSW values may eventually become more widely available from reference laboratories and allow for optimal antibiotic dosing, reducing antimicrobial resistance.

CFU = colony forming units; MIC = mean inhibitory concentration; MPC = mutant prevention concentration; MSW = mutant selection window

  1. Blondeau J. New concepts in antimicrobial susceptibility testing: The mutant prevention concentration and mutant selection window approach. Vet Derm 2009; 20:383-396.
  2. Xilin Zhao X, Drlica K. Restricting the selection of antibiotic-resistant mutants: A general strategy derived from fluoroquinolone studies. Clin Infect Dis 2001; 33(3):S147–S156.
  3. White SD. Review article: Systemic treatment of bacterial skin infections of dogs and cats. Vet Derm 1996; 7:133-143.



The perineum and nasal mucosa are primary colonization sites, and bacteria can be transferred to other body sites via licking and grooming.2

  • S pseudintermedius can be an opportunistic pathogen and create infection in dogs with underlying conditions that compromise the normal skin barrier or immune function.
  • Similar to S aureus, S pseudintermedius produces virulence factors to enhance infection, such as coagulase, proteases, thermonuclease, and toxins (ie, haemolysins, exfoliative toxins, enterotoxins). It also has the ability to bind to fibrinogen, fibronectin, and cytokeratin, as well as form biofilms.1

Underlying Conditions

Types of Conditions

Chronic or recurrent canine pyoderma is usually associated with an underlying cause; idiopathic pyoderma is rare. The most common underlying conditions include (Table 1):7

  • Hypersensitivity dermatitis
  • Parasitic skin infestations
  • Endocrinopathies
  • Follicular dysplasia
  • Keratinization disorders.


In one prospective study of 30 cases of canine recurrent pyoderma:8

  • Atopic dermatitis was found in 60% of cases. Atopic dogs are prone to recurrent skin infections due to increased adherence of staphylococcal bacteria to atopic canine skin cells,9-11 alterations in normal skin barrier function,12 and altered skin immune system function.13,14
  • Food allergy, flea allergy, and hypothyroidism each accounted for 7% of cases.
  • Hyperestrogenism, demodicosis, and zinc-responsive dermatosis each accounted for 4% of cases.
  • In only 2 dogs were underlying causes unidentified.

Identification of Conditions
Depending on clinical presentation, age of onset, seasonality, and other clinical signs, identification of underlying disorders includes:

  • Stringent flea control
  • Deep skin scrapings for Demodex
  • Trial treatment for scabies
  • Hypoallergenic diet trial
  • Intradermal allergy testing and desensitization
  • Screening for endocrinopathies
  • Skin biopsy for keratinization disorders.


  • In dogs without compromising disease, treatment of pyoderma will likely result in complete cure.
  • In patients with relapsing infection (recurs within 1–2 weeks), inadequate duration of treatment or bacterial resistance is likely.
  • In patients with recurrent infection (recurs within 3 months), identification/treatment of underlying disorders must be undertaken.7

Differential Diagnosis

The following differential diagnoses should be considered for any dog presenting with signs of pyoderma:

  • Demodicosis (Figure 1)
  • Dermatophytosis
  • Malassezia dermatitis
  • Noninfectious skin diseases (Figures 2 through 4), such as sebaceous adenitis, cutaneous lymphoma, and pemphigus foliaceus.



Clinical Signs

Canine pyoderma can be nonpruritic or minimally to markedly pruritic. Clinical signs can vary with the:

  • Depth of infection (superficial versus deep)
  • Location of infection (ie, haired versus nonhaired skin, mucocutaneous junctions, skin folds)
  • Breed of dog (in some cases).

The variable manifestations of pyoderma are listed in Tables 2 and 3. These tables will be repeated in the next article and will include figures and treatment recommendations.



Lesion Distribution & Appearance

Lesion distribution in cases of canine pyoderma:

  • May be localized or generalized
  • Can depend on the underlying cause
  • In pruritic dogs, lesions commonly occur in areas of self trauma and then spread
  • In dogs with endocrinopathies, lesions commonly begin on the trunk, then spread and become pruritic after development of infection.7

Clinical recognition of pyoderma can be complicated by differences in disease presentation, including area of the body affected and variations in coat length and breed of dog.


  • Mucocutaneous or nasal pyoderma can present as a crusting inflammatory disease similar to discoid lupus erythematosus (Figures 5–7).
  • Pyoderma on the thinly haired inguinal area may be easy to identify, but the same lesions on the thickly haired dorsal and lateral trunk may be harder to notice.
  • Short-coated dogs with folliculitis can present with raised tufts of hair on the trunk that can be confused with urticaria; however, lesions are not evanescent as occurs in urticarial lesions, and eventually hair is lost, leaving a moth-eaten appearance of the hair coat (Figure 8).
  • Long-coated dogs with pyoderma may initially demonstrate a dull coat with scaling +/- odor, easily epilated hair, and pruritus (Figure 9).
  • In chronic lesions of pyoderma, lichenification can develop that may appear identical to Malassezia infection (Figure 10).


Dog Breeds

Breed differences with regard to pyoderma manifestations are not uncommon (Figures 11–13).

  • Bulldogs often develop localized areas of plaque-like hyperkeratosis or papillomatous appearing dermatitis (Figure 11).
  • Dalmatians can develop small areas of brownish, discolored fur associated with papules (“dalmatian bronzing syndrome,” Figures 12 and 13).
  • Shar-peis often diffusely lose hair due to folliculitis, with minimal to no associated skin inflammation or visible papules.
  • Cocker spaniels may present with crusted plaques and follicular casting as a manifestation of pyoderma that can be mistaken for idiopathic or primary seborrhea (a far rarer diagnosis) and lead to inappropriate diagnosis and therapy.
  • Shetland sheep dogs often develop large superficial spreading areas of alopecia, erythema, and scaling.




Consideration of clinical signs and lesions is taken together with evaluation of microscopic cytology, skin scrapings, and possibly dermatophyte culture to eliminate other possible causes of folliculitis.



  • Impression cytology: Impression cytology of an exudative or lichenified lesion or a ruptured pustule directly onto a slide is easy to obtain.
  • Skin scrapings: Drier lesions, such as scaly areas or epidermal collarettes, can be sampled by collecting skin debris on a dull dry scalpel blade or spatula, smearing the debris on a slide, and then staining it with Diff-Quik or similar stain.
  • Tape preparation: Clear acetate tape preparation of an affected area can be applied onto a slide over a drop of blue stain.

Microscopic Evaluation

  • Slides are first scanned under 10× to identify areas of interest; then examined under 40× to 100× to evaluate inflammatory cells and organisms.
  • Bacterial folliculitis can be confirmed by identification of inflammatory cells and intracellular cocci (Figure 14).
  • In bacterial overgrowth syndrome, numerous extracellular bacteria are seen, which can include mixed flora with rod-shaped bacteria with or without Malassezia (Figures 15–16).
  • In cases of deep pyoderma, marked pyogranulomatous inflammation is usually seen, and organisms may be few in number (Figure 17).


Culture & Sensitivity

When to Culture

Culture and sensitivity of skin lesions associated with pyoderma is recommended if:

  • There is no clinical response and bacteria persist cytologically despite empiric antibiotic therapy (especially if a patient with recurrent pyoderma has been treated with multiple antibiotics) or if there is a history of multidrug- or methicillin-resistant infections
  • If primarily rod-shaped bacteria are found on cytology of lesions
  • In cases of deep pyoderma.7

Culture Techniques

Culture can be obtained with a sterile culturette via:

  • Swabbing contents of a freshly ruptured pustule
  • Swabbing debris or exudate under an intact crust or under the rim of an epidermal collarette
  • Obtaining a 4- to 6-mm punch biopsy of a papule or pustule for macerated aerobic tissue culture (the biopsy sample is placed in a red top tube with 0.25 mL sterile saline).

Since lidocaine has antibacterial activity, biopsies for culture should ideally be collected under sedation or general anesthesia.7

Sensitivity Testing

Sensitivity is usually tested by either:

  • Agar diffusion and disk process (ie, Kirby Bauer, which may be more indicative of topical antibiotic concentrations achievable at surface infections, such as otitis)
  • Broth diffusion technique, resulting in determination of the mean inhibitory concentration (MIC).

Antimicrobial susceptibility or resistance is then determined by comparing the measured MIC value to previously established breakpoints that take into account the drug’s in vitro activity, achievable and sustainable drug concentrations in the host, drug pharmacokinetics, and drug toxicity.15 See The Three Ms: MIC, MCP, & MSW for further information.


Biopsy of lesions is recommended if:

  • There is a poor response to antibiotics based on culture
  • No organisms are identified on cytology or culture
  • There is an unusual distribution (ie, facial, pinnal, or footpad lesions are suggestive of immune-mediated disease) or appearance of skin lesions.

Canine demodicosis can sometimes be difficult to find in very scarred, fibrotic areas, such as the feet or in the shar-pei breed, and may require biopsy for diagnosis.

The second article in this series will be published in our next issue and will focus on topical and systemic treatment of canine pyoderma.

MIC = mean inhibitory concentration


  1. Fitzgerald JR. The Staphylococcus intermedius group of bacterial pathogens: Species re-classification, pathogenesis and the emergence of methicillin resistance. Vet Derm 2009; 20:490-495.
  2. Frank LA, Kania SA, Hnilica KA, et al. Isolation of Staphylococcus schleiferi from dogs with pyoderma. JAVMA 2003; 222(4):451-454.
  3. May ER, Hnilica KA, Frank LA, et al. Isolation of Staphylococcus schleiferi from healthy dogs and dogs with otitis, pyoderma, or both. JAVMA 2005; 227(6): 928-931.
  4. Fazakerley J, Nuttall T, Sales D, et al. Staphylococcal colonization of mucosal and lesional skin sites in atopic and healthy dogs. Vet Derm 2009; 20:179-184.
  5. Harvey RG, Lloyd DH. Aspects of nasal, oropharyngeal and anal carriage of in normal dogs and dogs with pyoderma. Vet Derm 1998; 9(2):99-104.
  6. Griffeth GC, Morris DO, Abraham JL. Screening of skin carriage of methicillin-resistant coagulase-positive Staphylococci and Staphylococcus schleiferi in dogs with healthy and inflamed skin. Vet Derm 2008; 19(3):142-149.
  7. Scott DW, Miller WH, Griffin CE. Muller and Kirk’s Small Animal Dermatology, 6th ed. Philadelphia: WB Saunders, 2001, pp 291-296.
  8. Bensignor E, Germain PA. Canine recurrent pyoderma: A multicenter prospective study. Vet Derm 2004; 15(supp):42.
  9. McEwan NA. Adherence by Staphylococcus intermedius to canine keratinocytes in atopic dermatitis. Res Vet Sci 2000; 68:279-283.
  10. McEwan NA, Mellor D, Kalna G. Adherence by Staphylococcus intermedius to canine corneocytes: A preliminary study comparing noninflamed and inflamed atopic skin. Vet Derm 2006; 17:151-154.
  11. Simou C, Thoday KL, Forsyth PJ, et al. Adherence of Staphylococci to canine and human corneocytes: A preliminary study. Vet Derm 2005; 16:156-161.
  12. Olivry T. Is the skin barrier abnormal in dogs with atopic dermatitis? Vet Immunol Immunopath 2011; 144:11-16.
  13. McGirt LY, Beck LA. Innate immune defects in atopic dermatitis. J Allergy Clin Immunol 2006; 118:202-208.
  14. De Benedetto A, Agnihothri R, McGirt LY, et al. Atopic dermatitis: A disease caused by innate immune defects? J Invest Derm 2009; 129:14-30.
  15. White SD. Review article: Systemic treatment of bacterial skin infections of dogs and cats. Vet Derm 1996; 7:133-143.

f03_CoynerKimberly S. Coyner, DVM, Diplomate ACVD, received her DVM from Colorado State University. After a private referral practice internship and a year practicing emergency medicine, she completed a 2-year teaching position and then a 3-year residency position in dermatology at the University of Georgia. Dr. Coyner has authored several book chapters in the field of dermatology—Morgan’s Handbook of Small Animal Practice, 4th edition (2002) and Small Animal Dermatology Color Atlas and Therapeutic Guide (2001 and 2006)—as well as articles in Veterinary Dermatology, Veterinary Medicine, and The Compendium on Continuing Education for the Practicing Veterinarian. She is currently in private practice at the Dermatology Clinic for Animals of Las Vegas. Protection Status
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value='Cuba' >Cuba</option><option value='Curaçao' >Curaçao</option><option value='Cyprus' >Cyprus</option><option value='Czech Republic' >Czech Republic</option><option value='Denmark' >Denmark</option><option value='Djibouti' >Djibouti</option><option value='Dominica' >Dominica</option><option value='Dominican Republic' >Dominican Republic</option><option value='Ecuador' >Ecuador</option><option value='Egypt' >Egypt</option><option value='El Salvador' >El Salvador</option><option value='Equatorial Guinea' >Equatorial Guinea</option><option value='Eritrea' >Eritrea</option><option value='Estonia' >Estonia</option><option value='Eswatini (Swaziland)' >Eswatini (Swaziland)</option><option value='Ethiopia' >Ethiopia</option><option value='Falkland Islands' >Falkland Islands</option><option value='Faroe Islands' >Faroe Islands</option><option value='Fiji' >Fiji</option><option value='Finland' >Finland</option><option value='France' >France</option><option value='French Guiana' >French Guiana</option><option value='French Polynesia' >French Polynesia</option><option value='French Southern Territories' >French Southern Territories</option><option value='Gabon' >Gabon</option><option value='Gambia' >Gambia</option><option value='Georgia' >Georgia</option><option value='Germany' >Germany</option><option value='Ghana' >Ghana</option><option value='Gibraltar' >Gibraltar</option><option value='Greece' >Greece</option><option value='Greenland' >Greenland</option><option value='Grenada' >Grenada</option><option value='Guadeloupe' >Guadeloupe</option><option value='Guam' >Guam</option><option value='Guatemala' >Guatemala</option><option value='Guernsey' >Guernsey</option><option value='Guinea' >Guinea</option><option value='Guinea-Bissau' >Guinea-Bissau</option><option value='Guyana' >Guyana</option><option value='Haiti' >Haiti</option><option value='Heard and McDonald Islands' >Heard and McDonald Islands</option><option value='Holy See' >Holy See</option><option value='Honduras' >Honduras</option><option value='Hong Kong' >Hong Kong</option><option value='Hungary' >Hungary</option><option value='Iceland' >Iceland</option><option value='India' >India</option><option value='Indonesia' >Indonesia</option><option value='Iran' >Iran</option><option value='Iraq' >Iraq</option><option value='Ireland' >Ireland</option><option value='Isle of Man' >Isle of Man</option><option value='Israel' >Israel</option><option value='Italy' >Italy</option><option value='Jamaica' >Jamaica</option><option value='Japan' >Japan</option><option value='Jersey' >Jersey</option><option value='Jordan' >Jordan</option><option value='Kazakhstan' >Kazakhstan</option><option value='Kenya' >Kenya</option><option value='Kiribati' >Kiribati</option><option value='Kuwait' >Kuwait</option><option value='Kyrgyzstan' >Kyrgyzstan</option><option value='Lao People&#039;s Democratic Republic' >Lao People&#039;s Democratic Republic</option><option value='Latvia' >Latvia</option><option value='Lebanon' >Lebanon</option><option value='Lesotho' >Lesotho</option><option value='Liberia' >Liberia</option><option value='Libya' >Libya</option><option value='Liechtenstein' >Liechtenstein</option><option value='Lithuania' >Lithuania</option><option value='Luxembourg' >Luxembourg</option><option value='Macau' >Macau</option><option value='Macedonia' >Macedonia</option><option value='Madagascar' >Madagascar</option><option value='Malawi' >Malawi</option><option value='Malaysia' >Malaysia</option><option value='Maldives' >Maldives</option><option value='Mali' >Mali</option><option value='Malta' >Malta</option><option value='Marshall Islands' >Marshall Islands</option><option value='Martinique' >Martinique</option><option value='Mauritania' >Mauritania</option><option value='Mauritius' >Mauritius</option><option value='Mayotte' >Mayotte</option><option value='Mexico' >Mexico</option><option value='Micronesia' >Micronesia</option><option value='Moldova' >Moldova</option><option value='Monaco' >Monaco</option><option value='Mongolia' >Mongolia</option><option value='Montenegro' >Montenegro</option><option value='Montserrat' >Montserrat</option><option value='Morocco' >Morocco</option><option value='Mozambique' >Mozambique</option><option value='Myanmar' >Myanmar</option><option value='Namibia' >Namibia</option><option value='Nauru' >Nauru</option><option value='Nepal' >Nepal</option><option value='Netherlands' >Netherlands</option><option value='New Caledonia' >New Caledonia</option><option value='New Zealand' >New Zealand</option><option value='Nicaragua' >Nicaragua</option><option value='Niger' >Niger</option><option value='Nigeria' >Nigeria</option><option value='Niue' >Niue</option><option value='Norfolk Island' >Norfolk Island</option><option value='North Korea' >North Korea</option><option value='Northern Mariana Islands' >Northern Mariana Islands</option><option value='Norway' >Norway</option><option value='Oman' >Oman</option><option value='Pakistan' >Pakistan</option><option value='Palau' >Palau</option><option value='Palestine, State of' >Palestine, State of</option><option value='Panama' >Panama</option><option value='Papua New Guinea' >Papua New Guinea</option><option value='Paraguay' >Paraguay</option><option value='Peru' >Peru</option><option value='Philippines' >Philippines</option><option value='Pitcairn' >Pitcairn</option><option value='Poland' >Poland</option><option value='Portugal' >Portugal</option><option value='Puerto Rico' >Puerto Rico</option><option value='Qatar' >Qatar</option><option value='Réunion' >Réunion</option><option value='Romania' >Romania</option><option value='Russia' >Russia</option><option value='Rwanda' >Rwanda</option><option value='Saint Barthélemy' >Saint Barthélemy</option><option value='Saint Helena' >Saint Helena</option><option value='Saint Kitts and Nevis' >Saint Kitts and Nevis</option><option value='Saint Lucia' >Saint Lucia</option><option value='Saint Martin' >Saint Martin</option><option value='Saint Pierre and Miquelon' >Saint Pierre and Miquelon</option><option value='Saint Vincent and the Grenadines' >Saint Vincent and the Grenadines</option><option value='Samoa' >Samoa</option><option value='San Marino' >San Marino</option><option value='Sao Tome and Principe' >Sao Tome and Principe</option><option value='Saudi Arabia' >Saudi Arabia</option><option value='Senegal' >Senegal</option><option value='Serbia' >Serbia</option><option value='Seychelles' >Seychelles</option><option value='Sierra Leone' >Sierra Leone</option><option value='Singapore' >Singapore</option><option value='Sint Maarten' >Sint Maarten</option><option value='Slovakia' >Slovakia</option><option value='Slovenia' >Slovenia</option><option value='Solomon Islands' >Solomon Islands</option><option value='Somalia' >Somalia</option><option value='South Africa' >South Africa</option><option value='South Georgia' >South Georgia</option><option value='South Korea' >South Korea</option><option value='South Sudan' >South Sudan</option><option value='Spain' >Spain</option><option value='Sri Lanka' >Sri Lanka</option><option value='Sudan' >Sudan</option><option value='Suriname' >Suriname</option><option value='Svalbard and Jan Mayen Islands' >Svalbard and Jan Mayen Islands</option><option value='Sweden' >Sweden</option><option value='Switzerland' >Switzerland</option><option value='Syria' >Syria</option><option value='Taiwan' >Taiwan</option><option value='Tajikistan' >Tajikistan</option><option value='Tanzania' >Tanzania</option><option value='Thailand' >Thailand</option><option value='Timor-Leste' >Timor-Leste</option><option value='Togo' >Togo</option><option value='Tokelau' >Tokelau</option><option value='Tonga' >Tonga</option><option value='Trinidad and Tobago' >Trinidad and Tobago</option><option value='Tunisia' >Tunisia</option><option value='Turkey' >Turkey</option><option value='Turkmenistan' >Turkmenistan</option><option value='Turks and Caicos Islands' >Turks and Caicos Islands</option><option value='Tuvalu' >Tuvalu</option><option value='Uganda' >Uganda</option><option value='Ukraine' >Ukraine</option><option value='United Arab Emirates' >United Arab Emirates</option><option value='United Kingdom' >United Kingdom</option><option value='United States' >United States</option><option value='Uruguay' >Uruguay</option><option value='US Minor Outlying Islands' >US Minor Outlying Islands</option><option value='Uzbekistan' >Uzbekistan</option><option value='Vanuatu' >Vanuatu</option><option value='Venezuela' >Venezuela</option><option value='Vietnam' >Vietnam</option><option value='Virgin Islands, British' >Virgin Islands, British</option><option value='Virgin Islands, U.S.' >Virgin Islands, U.S.</option><option value='Wallis and Futuna' >Wallis and Futuna</option><option value='Western Sahara' >Western Sahara</option><option value='Yemen' >Yemen</option><option value='Zambia' >Zambia</option><option value='Zimbabwe' 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