Surgical Skills

Creating a Leak-Proof Ligature with Confidence, Part 1: Overview of Ligation & Surgical Binding Knots

Creating a Leak-Proof Ligature with Confidence, Part 1: Overview of Ligation & Surgical Binding Knots
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Daniel D. Smeak, DVM, Diplomate ACVS, and Kurtis M. Hazenfield, DVM, MS
Colorado State University

Certain binding, or friction, knots are highly useful in achieving consistently secure ligations, and ideal qualities of a first-throw friction knot include the ability to:

  • Cinch down tightly and completely without premature binding
  • Resist loosening once placed, allowing time for additional throws to be performed for a permanent secure knot.

Very little information is available in the human and veterinary literature describing indications and contraindications for use of friction knots for ligation, and we could find no published, well-illustrated instructions on how to tie these knots. There is also considerable confusion regarding the descriptions on tying, and correct names for, these knots.1

The goal of this article series is to review principles surgeons use to achieve hemostasis on blood vessels (or pedicles) and factors related to ligation security, and provide step-by-step instructions on how to create friction knots.

IMPORTANCE OF LIGATION

Ligation is the act of placing a ligature, and a ligature has 2 components:

  • Material that encircles tissue
  • Knot that secures the material in place.

For surgery, ligatures are used to tightly occlude blood vessels.2,3 Ligation of solitary vessels, or multiple vessels within a pedicle (mass ligation), is used virtually daily in practice for ovariohysterectomy, castration, amputation, splenectomy, and lobectomy.

Despite the introduction of newer electrosealing devices4-6 for use in open and minimally invasive surgery involving large vessels, ligatures are still considered the gold standard method to achieve hemostasis. Therefore, creating secure ligations is one of the most critical steps in most soft tissue surgeries.

Screen Shot 2015-05-14 at 2.56.06 PM

SUTURE SELECTION & SECURITY

Ideal Suture Selection

Generally speaking, it is good practice to choose the smallest suture material size that provides sufficient strength for the intended ligation.

Although many different suture materials can be used successfully to ligate a pedicle or critical blood vessel, many surgeons choose strong, absorbable monofilament material of 0 to 3-0 size for most small animal applications, such as the suture material listed in Table 1.2

Within the recommended suture size range listed, larger size suture materials are generally chosen for more substantial vessels or bulky pedicles. For larger arteries, some surgeons still recommend monofilament nonabsorbable suture material, such as polypropylene.

Other Suture Materials

Multifilament sutures, such as silk—a suture noted for its excellent handling and ligation qualities—can be used successfully for ligation, but:

  • Have been shown to increase risk of suture-related wound infections7
  • Tend to bind prematurely during tying: the first throw of the knot may not tighten fully around the vessel or pedicle because the suture develops friction among its strands.

Chromic catgut sutures have fallen out of favor for ligation because:

  • The suture is much weaker than the aforementioned materials, even after careful knotting
  • Within standard square knots, the suture often shows signs of fraying, which weakens the ligation.8

Therefore, if friction knots are used as part of ligature, chromic catgut is contraindicated due to the risk of excessive abrasion and fraying of the multifilament strands during tightening.

Testing Ligation Security

One of the most common, but dangerous ways, surgeons attempt to determine whether a ligation is leak-proof is to carefully and slowly release the clamps and any tension on the ligated pedicle; then watch to see whether bleeding occurs.

However, this method can be unsuccessful because:

  1. Simply crushing the vessel or pedicle with hemostatic forceps may only temporarily stop bleeding, particularly in hypothermic and/or somewhat hypotensive patients.
  2. Any tension on the pedicle may temporarily occlude flow through the vessel, giving the false impression that “all is well” at the site.
  3. Any fragile clot at the ligated site or crushed area can dislodge with extra blood flow as the patient is warmed, hypotensive effects of the anesthetic drugs wear off, and blood pressure rises.
  4. The ligature may appear to be tight, but only a millimeter or 2 of loosening of the knot can create a potentially life-threatening situation.

Unfortunately, especially when the ligature knot crushes deep within the pedicle during tightening, it is quite difficult to know for sure that safe and permanent hemostasis has been achieved. Most seasoned surgeons have experienced a latent bleed after a splenectomy or lobectomy, even though there was absolutely no evidence of bleeding from any pedicle at the time of approach closure.

Seasoned, meticulous surgeons safely practice the principles of secure ligation (Table 2) rather than relying solely on whether the pedicle bleeds soon after the ligation is completed.

Screen Shot 2015-05-14 at 2.57.09 PM

FIgure 1 Surgeons Knot

FIGURE 1. Surgeon’s knot.

Figure 4H DRHH FInal push

FIGURE 2. Double reverse half hitch knot.

Figure 5E Miller final tension

FIGURE 3. Traditional miller’s knot.

Figure 7C Strangle final knot final

FIGURE 4. Strangle knot.

BINDING (FRICTION) KNOTS

Binding knots, also called friction knots, are knots on a strand that either constrict a single object or hold multiple objects snugly together; the ends of the strands are either joined together or tucked under the turns of the knot. These knots are held in place by either:9

  •  Friction between the windings of line
  • Two ends of the line being knotted together.

During surgery, once the first friction knot/throw is applied and tightened firmly, it should be considered only temporarily stable; additional square throws are applied on top of it to make it permanently secure. In most cases, 3 to 4 snug additional square throws will secure the ligature knot.

Whippings, seizings, and lashings serve a similar purpose to binding knots, but contain too many wraps to be properly called a knot.

History of Binding Knots

Originally, these knots were designed for use by solo field workers to firmly close the end of burlap sacks. Binding knots were chosen because they would temporarily hold the neck of the sack tight without assistance until the knot was permanently secured with additional square throws.

Some of the more common friction knots used at that time were the miller’s, constrictor, strangle, double reverse half hitch, and surgeon’s knots.1,9

Why Not This Knot?

The surgeon’s throw can be used for ligation in practice, but it is generally NOT recommended for this use because:

  • This knot can bind prematurely, allowing the surgeon to falsely believe the knot has been tightly applied when it has not.
  • When the strands are tensioned and, as the encircled tissue is tapered, the double twisted throw tends to bind and resists further tightening.3
  • Compared with other friction knots, it has been shown to be the least able to resist loosening when placed under expansile force.10

1-Pass or 2-Pass Friction Knots

Friction knots commonly employed in veterinary surgery can be classified by how many passes are placed around the pedicle.

Some surgeons choose a 1-pass friction knot as it is easier to apply, requiring only 1 pass of the suture around the pedicle or vessel. One-pass friction knots include the surgeon’s knot (Figure 1), which is not recommended for ligation, and the double reverse half hitch knot (Figure 2).

Two-pass friction knots take a bit more effort to pass twice around the pedicle; however, the first throw effectively resists loosening. The highly dependable 2-pass friction knots commonly chosen in practice include the traditional miller’s knot (Figure 3) and the newly introduced strangle knot (Figure 4).9,10

IN SUMMARY

Ideally, a tightly placed friction knot will remain tight around a pedicle until subsequent square throws are completed, and this permanently locks the tight ligature knot in place, safely maintaining hemostasis. In Part 2 of this article series, step-by-step instructions and images will provide guidance on how to tie surgical ligature friction knots.

Photos courtesy of Charlie Kerlee, Medical Photographer, Colorado State University.

References

  1. Hardie RJ. Surgery STAT: Don’t forget the Miller’s knot. Available at veterinarynews.dvm360.com/surgery-stat-dont-forget-millers-knot.
  2. Toombs JP, Clarke KM. Basic operative techniques. In Slatter D (ed): Textbook of Small Animal Surgery, 3rd ed. Philadelphia: Saunders, 2003, pp 208-212.
  3. Knecht CD, Allen AR, Williams DJ, Johnson JH. Suture materials. Fundamental Techniques in Veterinary Surgery. 3rd ed. Philadelphia: Saunders, 1987, pp 28-49.
  4. Nezhat CN, Lewis M, King LP. Laparoscopic vessel sealing devices. Available at laparoscopy.blogs.com/prevention_management_3/2010/10/laparoscopic-vessel-sealing-devices.html.
  5. Newcomb WL, Hope WW, Schmeizer TM, et al. Comparison of blood vessel sealing among new electrosurgical and ultrasonic devices. Surg Endosc 2009; 23(1):90-96.
  6. Lamberton GR, Hsi RS, Jin DH, et al. Prospective comparison of four laparoscopic vessel ligation devices. J Endourol 2008; 22(10):2307-2312.
  7. Postlethwait RW, Dillon ML, Reeves JW. Experimental study of silk suture. Arch Surg 1962; 84:698-702.
  8. von Fraunhofer JA, Storey RS, Stone IK, Masterson BJ. Tensile strength of suture materials. J Biomed Mater Res 1985; 19(5):595-600.
  9. Ashley CW. The Ashley Book of Knots. New York: Doubleday, 1944, pp 11-20, 219, 597-599.
  10. Hazenfield K, Smeak DD. In vitro holding security of six friction knots used as a first throw in the creation of a vascular ligation. JAVMA 2014; 245(5):571-577.

Daniel D. SmeakDaniel D. Smeak, DVM, Diplomate ACVS, a soft tissue surgeon, is professor and Chief of Surgery and Oral Surgery at Colorado State University. He has written clinical and research articles, as well as textbook chapters on soft tissue surgery and core surgical skills instruction. His passion is teaching, and he is currently creating a complete set of interactive web-based core surgical skills modules to help train future veterinary students and practitioners around the world.

 

Kurtis M. HazenfieldKurtis M. Hazenfield, DVM, MS, is a surgeon at VCA Mission Animal Referral and Emergency Center in Mission, Kansas. His research on suture knot security was completed while serving as a chief resident in the Small Animal Surgery Section at Colorado State University. He has a passion for teaching, particularly surgical anatomy and basic surgical skills and principles. He received his MS in clinical sciences and surgery, and DVM at Colorado State University.

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value='Monaco' >Monaco</option><option value='Mongolia' >Mongolia</option><option value='Montenegro' >Montenegro</option><option value='Montserrat' >Montserrat</option><option value='Morocco' >Morocco</option><option value='Mozambique' >Mozambique</option><option value='Myanmar' >Myanmar</option><option value='Namibia' >Namibia</option><option value='Nauru' >Nauru</option><option value='Nepal' >Nepal</option><option value='Netherlands' >Netherlands</option><option value='New Caledonia' >New Caledonia</option><option value='New Zealand' >New Zealand</option><option value='Nicaragua' >Nicaragua</option><option value='Niger' >Niger</option><option value='Nigeria' >Nigeria</option><option value='Niue' >Niue</option><option value='Norfolk Island' >Norfolk Island</option><option value='North Korea' >North Korea</option><option value='Northern Mariana Islands' >Northern Mariana Islands</option><option value='Norway' >Norway</option><option value='Oman' >Oman</option><option value='Pakistan' >Pakistan</option><option value='Palau' >Palau</option><option value='Palestine, State of' >Palestine, State of</option><option value='Panama' >Panama</option><option value='Papua New Guinea' >Papua New Guinea</option><option value='Paraguay' >Paraguay</option><option value='Peru' >Peru</option><option value='Philippines' >Philippines</option><option value='Pitcairn' >Pitcairn</option><option value='Poland' >Poland</option><option value='Portugal' >Portugal</option><option value='Puerto Rico' >Puerto Rico</option><option value='Qatar' >Qatar</option><option value='Réunion' >Réunion</option><option value='Romania' >Romania</option><option value='Russia' >Russia</option><option value='Rwanda' >Rwanda</option><option value='Saint Barthélemy' >Saint Barthélemy</option><option value='Saint Helena' >Saint Helena</option><option value='Saint Kitts and Nevis' >Saint Kitts and Nevis</option><option value='Saint Lucia' >Saint Lucia</option><option value='Saint Martin' >Saint Martin</option><option value='Saint Pierre and Miquelon' >Saint Pierre and Miquelon</option><option value='Saint Vincent and the Grenadines' >Saint Vincent and the Grenadines</option><option value='Samoa' >Samoa</option><option value='San Marino' >San Marino</option><option value='Sao Tome and Principe' >Sao Tome and Principe</option><option value='Saudi Arabia' >Saudi Arabia</option><option value='Senegal' >Senegal</option><option value='Serbia' >Serbia</option><option value='Seychelles' >Seychelles</option><option value='Sierra Leone' >Sierra Leone</option><option value='Singapore' >Singapore</option><option value='Sint Maarten' >Sint Maarten</option><option value='Slovakia' >Slovakia</option><option value='Slovenia' >Slovenia</option><option value='Solomon Islands' >Solomon Islands</option><option value='Somalia' >Somalia</option><option value='South Africa' >South Africa</option><option value='South Georgia' >South Georgia</option><option value='South Korea' >South Korea</option><option value='South Sudan' >South Sudan</option><option value='Spain' >Spain</option><option value='Sri Lanka' >Sri Lanka</option><option value='Sudan' >Sudan</option><option value='Suriname' >Suriname</option><option value='Svalbard and Jan Mayen Islands' >Svalbard and Jan Mayen Islands</option><option value='Sweden' >Sweden</option><option value='Switzerland' >Switzerland</option><option value='Syria' >Syria</option><option value='Taiwan' >Taiwan</option><option value='Tajikistan' >Tajikistan</option><option value='Tanzania' >Tanzania</option><option value='Thailand' >Thailand</option><option value='Timor-Leste' >Timor-Leste</option><option value='Togo' >Togo</option><option value='Tokelau' >Tokelau</option><option value='Tonga' >Tonga</option><option value='Trinidad and Tobago' >Trinidad and Tobago</option><option value='Tunisia' >Tunisia</option><option value='Turkey' >Turkey</option><option value='Turkmenistan' >Turkmenistan</option><option value='Turks and Caicos Islands' >Turks and Caicos Islands</option><option value='Tuvalu' >Tuvalu</option><option value='Uganda' >Uganda</option><option value='Ukraine' >Ukraine</option><option value='United Arab Emirates' >United Arab Emirates</option><option value='United Kingdom' >United Kingdom</option><option value='United States' >United States</option><option value='Uruguay' >Uruguay</option><option value='US Minor Outlying Islands' >US Minor Outlying Islands</option><option value='Uzbekistan' >Uzbekistan</option><option value='Vanuatu' >Vanuatu</option><option value='Venezuela' >Venezuela</option><option value='Vietnam' >Vietnam</option><option value='Virgin Islands, British' >Virgin Islands, British</option><option value='Virgin Islands, U.S.' >Virgin Islands, U.S.</option><option value='Wallis and Futuna' >Wallis and Futuna</option><option value='Western Sahara' >Western Sahara</option><option value='Yemen' >Yemen</option><option value='Zambia' >Zambia</option><option value='Zimbabwe' 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