Parasitology , Practical Parasitology , Small Animal/Exotic Medicine

Heartworm Infection in Ferrets

Heartworm Infection in Ferrets
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Leonie Kondert, DVM
Joerg Mayer, DVM, MS, DABVP, DACZM
University of Georgia

Because of their friendly and playful character, ferrets are commonly kept as pets in the United States. Ferrets (Mustela putorius furo) belong to the family Mustelidae. The genus Mustela includes weasels, the European mink, the American mink, ferrets, and South American weasels. Ferrets were originally domesticated and bred to hunt rabbits; although this practice is not common in the United States, it is still in use in Europe and Asia.1 Ferrets, like more than 30 other mammalian species with documented infections, are susceptible to infection with heartworms (FIGURE 1).2

FIGURE 1. The right chamber of a ferret heart affected with Dirofilaria. Image courtesy of pathology service at the University of Georgia (UGA).

 

 

 

 

 

 

 

 

ETIOLOGY OF HEARTWORM DISEASE

The first description of a heartworm infection was written in Italy in 1626; the first description in the United States was in 1847.3 Heartworm disease (HWD) is a vector-borne disease caused by the filarial nematode Dirofilaria immitis. This parasite belongs to the superfamily Filarioidea and the family Onchocercidae.

Transmission is primarily via 3 species of mosquitos (Aedes trivittatus, Aedes sierrensis, and Culex quinquefasciatus); however, 70 species of mosquitos can function as vectors for infection.

PREVALENCE

HWD is widespread and can be found in North America, South America, Europe, Asia, and Australia.4,5 New models have been developed to predict HWD prevalence in dogs based on several factors (climate, geography, and society) and help in predicting outbreaks in the United States. The southeastern US has an exceptionally high risk of infection, but infections are described in all of the lower 48 states.6

Infections with D immitis in ferrets are described in laboratory settings with a susceptibility of 100%.7 Multiple cases of HWD in client-owned ferrets have been reported; however, compared to cats and dogs, there is not as much data regarding prevalence and susceptibility for HWD in ferrets.8 Housing a ferret outside is not recommended in areas where heartworm prevalence in cats and dogs is high. Even housing a ferret indoors 100% of the time is not a guarantee that it will avoid infection. One study found that about 25% of indoor cats have HWD.9

CLINICAL SIGNS

The clinical signs described in ferrets depend on the stage of HWD but are often associated with heart failure (FIGURE 2). Signs include dullness, anorexia, coughing, dyspnea, systolic heart murmur, pleural effusion, ascites, anemia, intravascular hemolysis, and acute renal and hepatic failure.1


FIGURE 2. A necropsy of a ferret affected with Dirofilaria. Note the globoid shape of the heart. Image courtesy of pathology service at UGA.

 

 

 

 

 

 

 

 

Bilirubinuria is a frequently described sign. In a study of clinical observations of naturally occurring HWD in ferrets, bilirubinuria was observed in 83% of the cases.10 A rare case of an aberrant larval migration infesting the subdural space of the cranial cavity was reported in Europe in 2010.11

HWD may be misidentified in ferrets. A study in 2008 showed HWD was diagnosed in 11 black-footed ferrets using an antigen-based enzyme-linked immunosorbent assay (ELISA). Polymerase chain reaction (PCR) amplification of the 5S spacer region of rDNA of the filarial sequences showed only 76% conformity with D immitis; it was reported that the population was infected with an undescribed filarial species.12

Pathogenesis

The life cycle of D immitis is 210 to 270 days. Female mosquitos can be infected with microfilariae during feeding on an infected host. After ingestion, the microfilariae transform into first-stage larvae (L1). Depending on temperature, L1 molt into infective third-stage larvae (L3) within about 10 to 14 days. The infective L3 are deposited on the skin of a potential host by the feeding mosquito. They enter the subcutaneous tissue of the host through the bite wound and, after a few days, transform into fourth-stage larvae (L4).

The L4 migrate through the subcutaneous and muscle tissue toward the thorax, where they molt to juvenile worms 50 to 70 days postinfection. The immature worms enter the bloodstream via peripheral veins and finally infest the pulmonary vasculature. A mean duration of 70 days after inoculation has been described for this migration. The juvenile worms reach maturity in the pulmonary arteries 180 days after infection. Mating takes place in the pulmonary arteries, and microfilariae are found in circulation at approximately 180 to 210 days after initial infection.3 Ferrets can be severely affected by a low heartworm burden of 1 to 2 adult worms.13

DIAGNOSTICS

Results of tests using antigen detection of a glycoprotein secreted by female heartworms will be falsely negative in the case of a male-only infection. Screening by blood test is not reliable in ferrets; symptomatic animals should be radiographed to evaluate the cardiac silhouette as the first step (FIGURE 3). A biochemistry profile and a complete blood cell count should always be performed. Changes commonly seen include elevated liver enzymes, azotemia, bilirubinemia, eosinophilia, basophilia, neutrophilia, nonregenerative anemia, and thrombocytopenia.3

FIGURE 3. Lateral thoracic radiograph of the thorax of a ferret. A globoid cardiac silhouette is a common finding in thoracic radiographs of affected animals. Image courtesy of radiology service at UGA.

 

 

 

 

 

 

 

 

If D immitis microfilariae are observed on a blood smear (FIGURE 4), the diagnosis of HWD is definitive. However, the Difil (Vetoquinol.com) or modified Knott’s test can have false-negative results owing to the normally low levels and transient nature of the microfilaremia in ferrets.

FIGURE 4. D immitis microfilaria in a canine blood smear. Image courtesy of Dr. Megan Caudill, UGA.

 

 

 

 

 

 

 

 

Housing a ferret outside is not recommended in areas where heartworm prevalence in cats and dogs is high. Even housing a ferret indoors 100% of the time is not a guarantee that it will avoid infection. In addition to medical prevention of HWD, housing the ferret in a mosquito-free environment is highly desirable.

Molecular genetic structures can be detected on PCR.3 D immitis can be observed via echocardiography as abnormal hyperechoic structures in the right atrium and/or right ventricle.14 Nonselective angiography provides a sensitive method of detection of adult heartworms in the cranial vena cava.15

TREATMENT

The decision of which treatment is most suitable for the patient should be based on whether the patient is in heart failure. Stabilization of a ferret in heart failure is similar to that for a cat or dog, administering the following as necessary:

  • Oxygen supplementation
  • Tranquilization of the patient with midazolam 0.1 to 0.3 mg/kg IM8
  • Furosemide 1 to 4 mg/kg PO, IM, or IV q6–24h13
  • Benazepril 0.25 to 0.5 mg/kg PO q24h8
  • Pimobendan 0.5 to 1.25 mg/kg PO q12h8

Once the patient is stable, the decision to pursue a surgical approach or medical management must be made (TABLE 1). All the published treatment options in ferrets consist of extralabel protocols. Alleviation of clinical signs with prednisone 0.5 mg/kg PO q12h can be elected if the patient is not stable enough for more invasive treatment.14

TABLE 1 Heartworm Treatment Options for Ferrets

THERAPYMEDICATION PROTOCOLHEALTH RISK
Conservative: Slow kill adulticidePrednisone 0.5 mg/kg PO q12h Ivermectin 0.05 - 0.2 mg/kg IM every 30 daysLow
Conservative: Slow kill adulticideMoxidectin 0.17 mg/ferret SC every 30 daysLow
AdulticideMelarsomine dihydrochloride 2.5 - 3.25 mg/kg IM on day 0, day 1, and day 31High
SurgeryTransvenous extraction of adult heartwormsHigh

Successful transvenous extraction of adult heartworms has been described in a 10-month-old ferret.16 After the surgery, the patient was medically managed with corticosteroids and a monthly heartworm preventive.16

If a more conservative approach is warranted, different protocols have been studied. Protocols using melarsomine 2.5 to 3.25 mg/kg IM have been described using a 2- or 3-injection protocol similar to that used in treating dogs. To prevent pain associated with the injections, sedation or anesthesia of the patient is necessary. Strict cage rest is indicated after treatment, as there is a high risk of thromboembolism.

The use of a combination of ivermectin 0.05 to 0.2 mg/kg IM every 30 days and prednisone 0.5 mg/kg PO q12h is indicated if the patient has a mild form of infection.13

Long-term treatment with moxidectin is described to be associated with fewer risks and can result in antigen-negative patients. In a study performed on 10 naturally infected ferrets, moxidectin was used at a dose of 0.17 mg/ferret SC, and 4 ferrets were reported to be antigen-negative after treatment.17 Because of the small sample size, we hesitate to recommend this protocol as a successful treatment option before further investigation has been performed. However, the longer course of treatment may result in greater endothelial damage from the prolonged presence of the parasites.18

PREVENTIVE MEDICINE

There are several options for medical prevention of HWD in ferrets (TABLE 2). The use of ivermectin 0.05 to 0.2 mg/kg PO every 30 days, milbemycin oxime 1.15 to 2.33 mg/kg PO every 30 days, or selamectin 6 to 18 mg/kg topically every 30 days is described.8,10,20 In one study, a combination of 10% imidacloprid and 1% moxidectin 0.4 mL/ferret (up to 4 kg) topically every 30 days was a successful preventative.21

TABLE 2 Heartworm Preventives For Ferrets

MEDICATIONDOSAGE
Ivermectin0.05 - 0.2 mg/kg PO every 30 days
Milbemycin oxime1.15 - 2.33 mg/kg PO every 30 days
Selamectin*6 - 18 mg/kg topical every 30 days
10% Imidacloprid + 1% moxidectin0.4 mL/ferret (up to 4kg) topically every 30 days
*Selamectin is considered a very safe preventive; even at a high dose of 35 mg/kg, no side effects have been reported in ferrets.19

In addition to medical prevention of HWD, housing the ferret in a mosquito-free environment is highly desirable.1

D immitis resistant to macrocyclic lactones have been described in the US Mississippi Delta.5 Therefore, patients receiving macrocyclic lactones to treat HWD should be screened annually for circulating microfilariae.3 However, since circulating microfilariae are not always present in an infected ferret, this screening method must be used with caution.

SUMMARY

The diagnosis and management of HWD in ferrets is not as straightforward as in dogs and cats for a variety of reasons. Unfortunately, there are fewer data available for ferrets regarding management of this life-threatening disease, and we hope more data will become available from cases managed in different clinics across the country.

As with other pets, prevention of HWD is recommended. Although the vast majority of ferrets are housed indoors, a preventive approach should be implemented year-round, especially in endemic areas.

References

  1. Mayer J, Marini RP, Fox JG. Biology and diseases of ferrets. In: Anderson LC, Otto G, Pritchett-Coming KR, et al, eds. Laboratory Animal Medicine. San Diego: Academic Press; 2015:577-622.
  2. Wang D, Bowman DD, Brown HE, et al. Factors influencing U.S. canine heartworm (Dirofilaria immitis) prevalence. Parasites Vectors 2014;7(1):264-282.
  3. Nelson CT. Heartworm disease. In: Green CE, ed. Infectious Diseases of the Dog and Cat. 4th ed. St. Louis: Elsevier; 2012:865-877.
  4. Morchón R, Carretón E, Gonzalez-Miguel J, et al. Heartworm disease (Dirofilaria immitis) and their vectors in Europe–new distribution trends. Front Physiol 2012;3:196.
  5. Wolstenholme AJ, Evans CC, Jimenez PD, et al. The emergence of macrocyclic lactone resistance in the canine heartworm, Dirofilaria immitis. Parasitology 2015;142(10):1249-1259.
  6. Bowman DD, Liu Y, McMahan CS, et al. Forecasting United States heartworm Dirofilaria immitis prevalence in dogs. Parasites Vectors 2016;9:540-552.
  7. Campbell WC, Blair LS. Dirofilaria immitis: experimental infections in the ferret (Mustela putorius furo). J Parasitol 1978;64(1):119-122.
  8. Morrisey JK, Kraus MS. Cardiovascular and other diseases. In: Quesenberry KE, Carpenter JW, eds. Ferrets, Rabbits and Rodents Clinical Medicine and Surgery. 3rd ed. St. Louis: Elsevier; 2012:62-77.
  9. Atkins CE, DeFrancesco TC, Coats JR, et al. Heartworm infection in cats: 50 cases (1985-1997). JAVMA 2000;217(3):355-358.
  10. Antinoff N. Clinical observations in ferrets with naturally occurring heartworm disease, and preliminary evaluation of treatment with ivermectin with and without melarsomine. AHS Symposium; 2001:45-47.
  11. Molnár V, Pazar P, Rigo D, et al. Autochthonous Dirofilaria immitis infection in a ferret with aberrant larval migration in Europe. J Small Anim Pract 2010;51(7):393-396.
  12. Wisely SM, Howard J, Williams SA, et al. An unidentified filarial species and its impact on fitness in wild populations of the black-footed ferret (Mustela nigripes). J Wildl Dis 2008;44(1):53-64.
  13. Powers LV. Bacterial and parasitic diseases of ferrets. Vet Clin North Am Exot Anim Pract 2009;12(3):531-561.
  14. Sasai H, Kato K, Sasaki T, et al. Echocardiographic diagnosis of dirofilariasis in a ferret. J Small Anim Pract 2000;41(4):172-174.
  15. McCall JW. Dirofiliariasis in the dometic ferret. Clin Tech Small Anim Pract 1998;13(2):109-112.
  16. Bradbury C, Saunders AB, Heatley JJ, et al. Transvenous heartworm extraction in a ferret with caval syndrome. J Am Anim Hosp Assoc 2010;46(1):31-35.
  17. Cottrell DK. Use of moxidectin (ProHeart 6) as a heartworm adulticide in 4 ferrets. Exotic DVM 2004;5:9-12.
  18. Mehlhorn H, ed. Encyclopedic Reference of Parasitology: Diseases, Treatment, Therapy. 2nd ed. Berlin: Springer; 2001.
  19. Miller DS, Eagle RP, Zabel S, et al. Efficacy and safety of selamectin in the treatment of Otodectes cynotis infestation in domestic ferrets. Vet Rec 2006;159(22):748.
  20. Fisher M, Beck W, Hutchinson MJ. Efficacy and safety of selamectin (Stronghold/Revolution) used off-label in exotic pets. Intern J Appl Res Vet Med 2007;5(3):87-96.
  21. Schaper R, Heine J, Arther RG, et al. Imidacloprid plus moxidectin to prevent heartworm infection (Dirofilaria immitis) in ferrets. Parasitol Res 2007;101(Suppl 1):57-62.

Leonie Kondert, DVM, served as a Zoo and Exotic Medicine intern at the University of Georgia. Dr. Kondert received her DVM from the University of Veterinary Medicine in Vienna, Austria and successfully completed the ECVFG exam. She also completed one year of training in Zoological Pathology at the Research Institute of Wildlife Ecology and worked three years with small animals in private practice. Her research interests include small mammal medicine and surgery.

 

 

Joerg Mayer, DVM, MS, DABVP, DACZM, received his doctoral degree in veterinary medicine from the University of Budapest, Hungary. He moved to the United States for an internship in zoological medicine and surgery at the Roger Williams Park Zoo in Providence, Rhode Island. Dr. Mayer then attended the Royal Veterinary College in London, England, to study for his master’s degree in wild animal health. After 10 years at Tufts University as the head of the clinical service for exotic animals, he became an associate professor in zoological medicine at the University of Georgia. He lectures regularly at national and international conferences on all aspects of exotic animal medicine, and he has published many scientific articles and book chapters, some of which have been translated into French, Spanish, and Portuguese.

 

 

 

 

 

 

 

 

 

 

 

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value='Curaçao' >Curaçao</option><option value='Cyprus' >Cyprus</option><option value='Czech Republic' >Czech Republic</option><option value='Denmark' >Denmark</option><option value='Djibouti' >Djibouti</option><option value='Dominica' >Dominica</option><option value='Dominican Republic' >Dominican Republic</option><option value='Ecuador' >Ecuador</option><option value='Egypt' >Egypt</option><option value='El Salvador' >El Salvador</option><option value='Equatorial Guinea' >Equatorial Guinea</option><option value='Eritrea' >Eritrea</option><option value='Estonia' >Estonia</option><option value='Eswatini (Swaziland)' >Eswatini (Swaziland)</option><option value='Ethiopia' >Ethiopia</option><option value='Falkland Islands' >Falkland Islands</option><option value='Faroe Islands' >Faroe Islands</option><option value='Fiji' >Fiji</option><option value='Finland' >Finland</option><option value='France' >France</option><option value='French Guiana' >French Guiana</option><option value='French Polynesia' >French Polynesia</option><option value='French Southern Territories' >French Southern Territories</option><option value='Gabon' >Gabon</option><option value='Gambia' >Gambia</option><option value='Georgia' >Georgia</option><option value='Germany' >Germany</option><option value='Ghana' >Ghana</option><option value='Gibraltar' >Gibraltar</option><option value='Greece' >Greece</option><option value='Greenland' >Greenland</option><option value='Grenada' >Grenada</option><option value='Guadeloupe' >Guadeloupe</option><option value='Guam' >Guam</option><option value='Guatemala' >Guatemala</option><option value='Guernsey' >Guernsey</option><option value='Guinea' >Guinea</option><option value='Guinea-Bissau' >Guinea-Bissau</option><option value='Guyana' >Guyana</option><option value='Haiti' >Haiti</option><option value='Heard and McDonald Islands' >Heard and McDonald Islands</option><option value='Holy See' >Holy See</option><option value='Honduras' >Honduras</option><option value='Hong Kong' >Hong Kong</option><option value='Hungary' >Hungary</option><option value='Iceland' >Iceland</option><option value='India' >India</option><option value='Indonesia' >Indonesia</option><option value='Iran' >Iran</option><option value='Iraq' >Iraq</option><option value='Ireland' >Ireland</option><option value='Isle of Man' >Isle of Man</option><option value='Israel' >Israel</option><option value='Italy' >Italy</option><option value='Jamaica' >Jamaica</option><option value='Japan' >Japan</option><option value='Jersey' >Jersey</option><option value='Jordan' >Jordan</option><option value='Kazakhstan' >Kazakhstan</option><option value='Kenya' >Kenya</option><option value='Kiribati' >Kiribati</option><option value='Kuwait' >Kuwait</option><option value='Kyrgyzstan' >Kyrgyzstan</option><option value='Lao People&#039;s Democratic Republic' >Lao People&#039;s Democratic Republic</option><option value='Latvia' >Latvia</option><option value='Lebanon' >Lebanon</option><option value='Lesotho' >Lesotho</option><option value='Liberia' >Liberia</option><option value='Libya' >Libya</option><option value='Liechtenstein' >Liechtenstein</option><option value='Lithuania' >Lithuania</option><option value='Luxembourg' >Luxembourg</option><option value='Macau' >Macau</option><option value='Macedonia' >Macedonia</option><option value='Madagascar' >Madagascar</option><option value='Malawi' >Malawi</option><option value='Malaysia' >Malaysia</option><option value='Maldives' >Maldives</option><option value='Mali' >Mali</option><option value='Malta' >Malta</option><option value='Marshall Islands' >Marshall Islands</option><option value='Martinique' >Martinique</option><option value='Mauritania' >Mauritania</option><option value='Mauritius' >Mauritius</option><option value='Mayotte' >Mayotte</option><option value='Mexico' >Mexico</option><option value='Micronesia' >Micronesia</option><option value='Moldova' >Moldova</option><option value='Monaco' >Monaco</option><option value='Mongolia' >Mongolia</option><option value='Montenegro' >Montenegro</option><option value='Montserrat' >Montserrat</option><option value='Morocco' >Morocco</option><option value='Mozambique' >Mozambique</option><option value='Myanmar' >Myanmar</option><option value='Namibia' >Namibia</option><option value='Nauru' >Nauru</option><option value='Nepal' >Nepal</option><option value='Netherlands' >Netherlands</option><option value='New Caledonia' >New Caledonia</option><option value='New Zealand' >New Zealand</option><option value='Nicaragua' >Nicaragua</option><option value='Niger' >Niger</option><option value='Nigeria' >Nigeria</option><option value='Niue' >Niue</option><option value='Norfolk Island' >Norfolk Island</option><option value='North Korea' >North Korea</option><option value='Northern Mariana Islands' >Northern Mariana Islands</option><option value='Norway' >Norway</option><option value='Oman' >Oman</option><option value='Pakistan' >Pakistan</option><option value='Palau' >Palau</option><option value='Palestine, State of' >Palestine, State of</option><option value='Panama' >Panama</option><option value='Papua New Guinea' >Papua New Guinea</option><option value='Paraguay' >Paraguay</option><option value='Peru' >Peru</option><option value='Philippines' >Philippines</option><option value='Pitcairn' >Pitcairn</option><option value='Poland' >Poland</option><option value='Portugal' >Portugal</option><option value='Puerto Rico' >Puerto Rico</option><option value='Qatar' >Qatar</option><option value='Réunion' >Réunion</option><option value='Romania' >Romania</option><option value='Russia' >Russia</option><option value='Rwanda' >Rwanda</option><option value='Saint Barthélemy' >Saint Barthélemy</option><option value='Saint Helena' >Saint Helena</option><option value='Saint Kitts and Nevis' >Saint Kitts and Nevis</option><option value='Saint Lucia' >Saint Lucia</option><option value='Saint Martin' >Saint Martin</option><option value='Saint Pierre and Miquelon' >Saint Pierre and Miquelon</option><option value='Saint Vincent and the Grenadines' >Saint Vincent and the Grenadines</option><option value='Samoa' >Samoa</option><option value='San Marino' >San Marino</option><option value='Sao Tome and Principe' >Sao Tome and Principe</option><option value='Saudi Arabia' >Saudi Arabia</option><option value='Senegal' >Senegal</option><option value='Serbia' >Serbia</option><option value='Seychelles' >Seychelles</option><option value='Sierra Leone' >Sierra Leone</option><option value='Singapore' >Singapore</option><option value='Sint Maarten' >Sint Maarten</option><option value='Slovakia' >Slovakia</option><option value='Slovenia' >Slovenia</option><option value='Solomon Islands' >Solomon Islands</option><option value='Somalia' >Somalia</option><option value='South Africa' >South Africa</option><option value='South Georgia' >South Georgia</option><option value='South Korea' >South Korea</option><option value='South Sudan' >South Sudan</option><option value='Spain' >Spain</option><option value='Sri Lanka' >Sri Lanka</option><option value='Sudan' >Sudan</option><option value='Suriname' >Suriname</option><option value='Svalbard and Jan Mayen Islands' >Svalbard and Jan Mayen Islands</option><option value='Sweden' >Sweden</option><option value='Switzerland' >Switzerland</option><option value='Syria' >Syria</option><option value='Taiwan' >Taiwan</option><option value='Tajikistan' >Tajikistan</option><option value='Tanzania' >Tanzania</option><option value='Thailand' >Thailand</option><option value='Timor-Leste' >Timor-Leste</option><option value='Togo' >Togo</option><option value='Tokelau' >Tokelau</option><option value='Tonga' >Tonga</option><option value='Trinidad and Tobago' >Trinidad and Tobago</option><option value='Tunisia' >Tunisia</option><option value='Turkey' >Turkey</option><option value='Turkmenistan' >Turkmenistan</option><option value='Turks and Caicos Islands' >Turks and Caicos Islands</option><option value='Tuvalu' >Tuvalu</option><option value='Uganda' >Uganda</option><option value='Ukraine' >Ukraine</option><option value='United Arab Emirates' >United Arab Emirates</option><option value='United Kingdom' >United Kingdom</option><option value='United States' >United States</option><option value='Uruguay' >Uruguay</option><option value='US Minor Outlying Islands' >US Minor Outlying Islands</option><option value='Uzbekistan' >Uzbekistan</option><option value='Vanuatu' >Vanuatu</option><option value='Venezuela' >Venezuela</option><option value='Vietnam' >Vietnam</option><option value='Virgin Islands, British' >Virgin Islands, British</option><option value='Virgin Islands, U.S.' >Virgin Islands, U.S.</option><option value='Wallis and Futuna' >Wallis and Futuna</option><option value='Western Sahara' >Western Sahara</option><option value='Yemen' >Yemen</option><option value='Zambia' >Zambia</option><option value='Zimbabwe' >Zimbabwe</option></select> <label for='input_6_10_6' id='input_6_10_6_label' 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